Phosphorescence quenching and the microcirculation: An automated, multipoint oxygen tension measuring instrument

A new, phosphorescence‐based optical instrument for making oxygen tension (PO2) measurements in individual vessels of microvascular preparations is described. Palladium–porphyrin compounds injected into the systemic vasculature are excited by light and the measured phosphorescence emission lifetime...

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Published in:Review of scientific instruments 1995-10, Vol.66 (10), p.5075-5084
Main Authors: Shonat, Ross D., Richmond, Keith N., Johnson, Paul C.
Format: Article
Language:English
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cited_by cdi_FETCH-LOGICAL-c297t-66e0f98e2d8243aac88d85067f35730a7c416c2b9b74d981292d5f33346872f23
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creator Shonat, Ross D.
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description A new, phosphorescence‐based optical instrument for making oxygen tension (PO2) measurements in individual vessels of microvascular preparations is described. Palladium–porphyrin compounds injected into the systemic vasculature are excited by light and the measured phosphorescence emission lifetime used to calculate intravascular PO2. Because this technique is relatively new, we have undertaken a rigorous examination of its usefulness and inherent limitations for microvascular studies.
doi_str_mv 10.1063/1.1146133
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source AIP_美国物理联合会现刊（与NSTL共建）; AIP Digital Archive
title Phosphorescence quenching and the microcirculation: An automated, multipoint oxygen tension measuring instrument
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