A novel high-throughput scanning microscope for label-free detectionof protein and small-molecule chemical microarrays

We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10000 protein or...

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Bibliographic Details
Published in:Review of scientific instruments 2008-01, Vol.79 (1), p.013708-013708-7
Main Authors: Fei, Y. Y., Landry, J. P., Sun, Y. S., Zhu, X. D., Luo, J. T., Wang, X. B., Lam, K. S
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Summary:We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.
ISSN:0034-6748
1089-7623
DOI:10.1063/1.2830286