Deterministic separation of cancer cells from blood at 10 mL/min

Circulating tumor cells (CTCs) and circulating clusters of cancer and stromal cells have been identified in the blood of patients with malignant cancer and can be used as a diagnostic for disease severity, assess the efficacy of different treatment strategies and possibly determine the eventual loca...

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Bibliographic Details
Published in:AIP advances 2012-12, Vol.2 (4), p.42107-42107
Main Authors: Loutherback, Kevin, D'Silva, Joseph, Liu, Liyu, Wu, Amy, Austin, Robert H., Sturm, James C.
Format: Article
Language:English
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Regular
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Summary:Circulating tumor cells (CTCs) and circulating clusters of cancer and stromal cells have been identified in the blood of patients with malignant cancer and can be used as a diagnostic for disease severity, assess the efficacy of different treatment strategies and possibly determine the eventual location of metastatic invasions for possible treatment. There is thus a critical need to isolate, propagate and characterize viable CTCs and clusters of cancer cells with their associated stroma cells. Here, we present a microfluidic device for mL/min flow rate, continuous-flow capture of viable CTCs from blood using deterministic lateral displacement (DLD) arrays. We show here that a DLD array device can isolate CTCs from blood with capture efficiency greater than 85% CTCs at volumetric flow rates of up to 10 mL/min with no effect on cell viability.
ISSN:2158-3226
2158-3226
DOI:10.1063/1.4758131