Characterization of partial DREB and SOD genes from three Bali local rice cultivars

Rice is a staple food for many Asian countries including Indonesia. High human population growth, reduction of the fertile field and water source resulted in a limitation of rice production. These conditions promote the development of rice that resistant to drought. Evaluation of characters that con...

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Bibliographic Details
Main Authors: Pharmawati, Made, Kurniasih, Eka Maya
Format: Conference Proceeding
Language:English
Subjects:
Adaptation
Cultivars
Dehydration
Deoxyribonucleic acid
DNA
Drought
Electrophoresis
Gene expression
Genes
Population growth
Rice
Superoxide dismutase
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Summary:Rice is a staple food for many Asian countries including Indonesia. High human population growth, reduction of the fertile field and water source resulted in a limitation of rice production. These conditions promote the development of rice that resistant to drought. Evaluation of characters that contribute to adaptation strategy of rice to drought is important in rice breeding. The adaptation process involves the expression of genes specific such as DREB (dehydration responsive element binding) and SOD (superoxide dismutase). This research aims to provide basic information on the sequence of DREB and SOD genes from Bali local rice cultivars for later study of rice drought resistance. Three cultivars of Bali local rice namely “Mansur A”, “Merah Cendana” and “Putih Cempaka” were used in this study. Seedlings were grown hydroponically and leaves were harvested for DNA extraction at two weeks after transfer to a hydroponic system. PCR reactions were carried out using DREB and SOD specific primers. Agarose electrophoresis revealed a DREB fragment of 1020 bp. Sequence alignment showed identical DREB sequences between the three Bali rice cultivars. A neighbor-joining analysis of other DREB sequences showed that sequences from the Bali rice cultivars tested were very closely related to DREB1 of Oryza sativa Indica Group. PCR of SOD gene of the three Bali rice cultivars showed 970 bp fragments in agarose gel. Sequence relationships using Neighbor-Joining analysis showed that SOD gene sequence from the three Bali rice cultivars was closely related to Oryza sativa Japonica Group cytosolic cooper/zinc superoxide dismutase gene.
ISSN:0094-243X
1551-7616
DOI:10.1063/1.4953511