Development of extraction procedure for determination of mercury species using SPME-assisted dispersive derivative agent

• Main Authors: Abdullah, Md Pauzi, Khalik, Wan Mohd Afiq Wan Mohd, Othman, Mohamed Rozali
• Format: Conference Proceeding
• Language: English
• Subjects: Factorial design; Low level; Mathematical analysis; Reagents; Reproducibility; Solid phases
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/1.4966741

The extraction procedure for determination of low level mercury using solid phase microextraction was successfully carried out. Design of experimental works using factorial design and central composite design were applied to screen and predict the optimum condition for extraction step. In this study, variables namely concentration level (5 % m/v) and volume of derivatization solution (150 µL) has depicted as main effect for controlling the suitability of derivative reagent condition. Maximum of signal response (account as total peak areas for mercury species) was obtained when extraction procedure was set up at pH of water sample (5.8), extraction time (14 min), extraction temperature (43 °C) and stirring rate (450 rpm). Reducing time required to reach equilibrium is new improvement achieved in this study. Detection limit for each species (MeHg 26.17 ngL−1; EtHg 48.84 ngL−1 and IHg 14.11 ngL−1) was calculated lower than our previous work. Recovery, repeatability and reproducibility trial were recorded varied at acceptable range and relative standard deviation was calculated below than 10 %.