Phenol biodegradation by immobilized Pseudomonas putida FNCC-0071 cells in alginate beads

• Main Authors: Hakim, Lukman Nul, Rochmadi, Sutijan
• Format: Conference Proceeding
• Language: English
• Subjects: Acclimatization; Alginates; Beads; Biodegradation; Data transfer (computers); Dynamic models; Extrusion; Mass transfer; Parameters; Phenols; Pseudomonas putida; Reaction kinetics; Substrates
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/1.4985518

Phenol is one of industrial liquid waste which is harmful to the environment, so it must be degraded. It can be degraded by immobilized Pseudomonas putida FNCC-0071 cells. It needs the kinetics and mass transfer data to design this process which can be estimated by the proposed dynamic model in this study. This model involves simultaneous diffusion and reaction in the alginate bead and liquid bulk. The preliminary stage of phenol biodegradation process was acclimatization cells. This is the stage where cells were acclimated to phenol as carbon source (substrate). Then the acclimated cells were immobilized in alginate beads by extrusion method. The variation of the initial phenol concentration in the solution is 350 to 850 ppm where 60 g alginate bead contained by cells loaded into its solution in reactor batch, so then biodegradation occurs. In this study, the average radius of alginate bead was 0.152 cm. The occurred kinetic reaction process can be explained by Blanch kinetic model with the decreasing of parameter μmax’ while the increasing values of initial phenol concentration in the same time, but the parameters KM, KM’, and kt were increasing by the rising values of initial phenol concentration. The value of the parameter β is almost zero. Effective diffusivity of phenol and cells are 1.11 × 10−5±4.5% cm2 s−1 and 1.39 × 10−7± 0.04% cm2 s−1. The partition coefficient of phenol and cells are 0.39 ± 15% and 2.22 ± 18%.