Metal extraction studies of protein isolated from natural rubber latex (NRL) waste

Multiple rounds of centrifugation (MC) against ammonium sulphate purification (SP) were investigated for the interactions of the resulting proteins towards metal solutions. The ammonium sulphate purification method involved a large amount of ammonium sulphate and needed to be removed after the preci...

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Bibliographic Details
Main Authors: Tahir, Hairani, Misran, Misni, Mohamed, Rahmah
Format: Conference Proceeding
Language:English
Subjects:
Aluminum
Ammonium sulfate
Inductively coupled plasma
Latex
Lead
Natural rubber
Proteins
Purification
Sulfuric acid
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Summary:Multiple rounds of centrifugation (MC) against ammonium sulphate purification (SP) were investigated for the interactions of the resulting proteins towards metal solutions. The ammonium sulphate purification method involved a large amount of ammonium sulphate and needed to be removed after the precipitation process thus MC methods by centrifugation only were used. The metal extraction capabilities were attained using inductively coupled plasma (ICP). Overall data obtained showed that the extraction capabilities’ limiting factor was the amount of proteins used versus concentration of metal solution. With 0.2 g protein the percentage of removal can hold up to 60 % for 2 ppm metal solution whilst at 5 ppm the metal removal was reduced by 40 % and was further reduced as the metal concentration increased. On the other hand, multiple centrifugation method for metals such as lead, cadmium, and copper gave about the same percentage of metal uptake. However, zinc and aluminium can only work with ammonium sulphate purified proteins.
ISSN:0094-243X
1551-7616
DOI:10.1063/1.5047182