Evaluation of the potential of Nano Silver Fluoride against Streptococcus mutans and Enterobacter faecalis in various stages of biofilm maturation

• Main Authors: Gultom, Ferry P., Khoirunnisa, Novia, Sahlan, Muhamad, Soekanto, Sri Angky
• Format: Conference Proceeding
• Language: English
• Subjects: Bacteria; Biofilms; Fluorides; Maturation; Side effects; Viability
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/1.5096711

Silver Diamine Fluoride (SDF) is a liquid that has been proven to be an anti caries agent. Unfortunately, SDF leaves a black stain on the surface of the teeth where it was applicated, and it also has a metallic taste. Nano Silver Fluoride has the potential to replace Silver Diamine Fluoride as an anti caries agent without having the same side effects. To analyze the effect of NSF on the viability of Streptococcus mutans and Enterobacter faecalis in various phases of biofilm maturation compared to SDF, E. faecalis and S. mutans biofilm was incubated for 4 hours (adherence phase), 12 hours (active accumulation phase) and 24 hours (maturation phase) at 37 °C, then exposed to NSF at concentration (Ag+ 0,4%+F− 2,26%), (Ag+ 0,9%+F− 2,26%), (Ag+ 1,4%+F− 2,26%), (Ag+ 1,9%+F− 2,26%) for 1 hour. The percentage of viability was tested with MTT assay. Biofilm viability of E. faecalis and S. mutans in various phases showed no significant difference (p>0,05) in one-way ANOVA, compared to SDF. Results from this study showed that NSF was comparable with Silver Diamine Fluoride in inhibiting formation of E. faecalis and S. mutans biofilm.