Dependence of O. Vulgare extract and cold plasma on the formation of silver nanoparticles: Anticancer activity against L20B cells

• Main Authors: Mazhir, Sabah N., Ali, Iman A. Mohammed, Al-Ahmed, Hazim I., Bououdina, Mohamed, Ali, Firas A. Mohammed
• Format: Conference Proceeding
• Language: English
• Subjects: Anticancer properties; Antioxidants; Biotechnology; Cold plasmas; Color; Damage; Infrared spectroscopy; Nanoparticles; Optical properties; Particle size; Particle size distribution; Plasma; Silver; Tumors
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0000112

In this study, silver nanoparticles (AgNPs) were synthesized by adding Origanum Vulgar to silver nitrate (AgNO3) in addition to the use of cold plasma. The OV-Ag NPs formation process can be monitored once the color turns red as a base marker; the color change of the solution represents the withdrawal of nitrate salts from the compound and the occurrence of surface plasmids. Part of the NPs of cold plasma is a second extract that can be used in treatment. Factors such as size and shape characteristics, plasmodium surface, structure and optical properties were studied by UV spectroscopy, FE-SEM, FT-IR and AFM. The average particle size distribution is found identical for both (44.2 - 88.1) nm in (AgNPs)1, while the average particle size distribution was (44.2 - 86.1) nm in (AgNPs)2. Then, OV-Ag NPs were added to the L20B tumor cell lines. The results showed that NPs were effective in inhibiting human pathogens in the presence of (NPs)1. The inhibition efficacy was found to be better for (NPs)1 compared to (NPs)2, because of a combination in the compound after exposure to plasma energy. The results also showed that NPs response based on dose concentration used against L20B tumor cell lines. Antioxidants were also identified in all cell groups. In addition, the resulting damage rate was calculated in the DNA of L20B tumor cells. The rate of damage in the DNA of cancer cells increased after exposure. The results showed that NPs located on living cell lines gave better results than control.