Low-pass filtering compensation in common-path digital holographic microscopy

A low-pass filtering compensation (LPFC) method is proposed to compensate for phase aberrations in point diffraction-based common-path digital holographic microscopy. This method estimates the phase aberration from the object hologram by Fourier transform and low-pass spatial filtering. The estimate...

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Bibliographic Details
Published in:Applied physics letters 2020-09, Vol.117 (12)
Main Authors: Dong, Jie, Yetisen, Ali K., Dong, Xingchen, Pöller, Franziska, Jakobi, Martin, Liu, Zhanwei, Salazar Bloise, Félix, Koch, Alexander W.
Format: Article
Language:English
Subjects:
Aberration
Applied physics
Compensation
Fourier transforms
Low pass filters
Microscopy
Spatial filtering
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Summary:A low-pass filtering compensation (LPFC) method is proposed to compensate for phase aberrations in point diffraction-based common-path digital holographic microscopy. This method estimates the phase aberration from the object hologram by Fourier transform and low-pass spatial filtering. The estimated phase aberration is subtracted from the object phase image to achieve single-hologram phase compensation. The accuracy and capability of LPFC for phase compensation were demonstrated by experiments on a Ronchi grating and a human blood smear. LPFC provides phase compensation for both smooth objects and objects containing abrupt edges, in the special case of a system with relatively high-frequency objects and low-frequency slight phase aberrations. LPFC operates without the need for fitting procedures, iterative steps, or prior knowledge of the optical parameters, which substantially simplifies the process of phase compensation in quantitative phase imaging.
ISSN:0003-6951
1077-3118
DOI:10.1063/5.0019209