The analysis of the secondary metabolite compounds and antioxidant activity of ethyl acetate extracts from the stems of Bouea macrophylla griff

Gandaria (Bouea macrophylla Griff) is a potential source of antioxidant compounds and this study was conducted to analyze the secondary metabolite constituents and antioxidant activity of the ethyl acetate extracted from B. macrophylla stem. The extracts were isolated and fractioned using chromatogr...

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Main Authors: Rudiana, Tarso, Herisman, Dani, Stiani, Sofi Nurmay, Yusransyah, Suryani, Nani, Yohanes, Ricky, Noviany, Hadi, Sutopo
Format: Conference Proceeding
Language:English
Subjects:
Antioxidants
Chromatography
Column chromatography
Diethanolamide
Ethyl acetate
Liquid chromatography
Mass spectrometry
Metabolites
Stems
Thin layer chromatography
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creator Rudiana, Tarso
Herisman, Dani
Stiani, Sofi Nurmay
Yusransyah
Suryani, Nani
Yohanes, Ricky
Noviany
Hadi, Sutopo
description Gandaria (Bouea macrophylla Griff) is a potential source of antioxidant compounds and this study was conducted to analyze the secondary metabolite constituents and antioxidant activity of the ethyl acetate extracted from B. macrophylla stem. The extracts were isolated and fractioned using chromatography techniques such as thin-layer chromatography (TLC) and column chromatography (CC). Moreover, the composition of the secondary metabolites was determined by Liquid Chromatography-Mass Spectrometry (LC-MS) while the antioxidant property of the extracts and fractions was obtained using the 1,1-diphenyl-2-picrylhidrazil (DPPH) method. The ethyl acetate extract was separated with CC and 8 subfractions represented by codes E1-E8 were obtained with E1 subfraction observed to have the most active fraction on the antioxidant assay at a moderate activity with an IC50 value of 232.96 ppm. E1 was further analyzed with LC-MS and two major compounds found were lauric diethanolamide and loganine with their synergy effects suggested to be the reason for the subfraction’s moderate antioxidant activity. However, E1 requires purification to determine the most active antioxidant compound isolated from ethyl acetate extract of B. macrophylla stem.
doi_str_mv 10.1063/5.0062482
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source American Institute of Physics:Jisc Collections:Transitional Journals Agreement 2021-23 (Reading list)
subjects Antioxidants
Chromatography
Column chromatography
Diethanolamide
Ethyl acetate
Liquid chromatography
Mass spectrometry
Metabolites
Stems
Thin layer chromatography
title The analysis of the secondary metabolite compounds and antioxidant activity of ethyl acetate extracts from the stems of Bouea macrophylla griff
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