Transcription factor caprice as a target to induce anthocyanin biosynthesis in oilseed rape

CAPRICE (CPC) gene negatively regulates anthocyanin biosynthesis. It encodes a small single-repeat R3 MYB transcription factor, one of the seven of the kind, discovered in Arabidopsis. Oilseed rape (Brassica napus) is an economically valuable crop, a prospective object of genetic transformation and...

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Bibliographic Details
Main Authors: Mikhaylova, Elena, Khusnutdinov, Emil, Shein, Michael, Sukhareva, Anna, Panfilova, Maria, Kuluev, Bulat
Format: Conference Proceeding
Language:English
Subjects:
Anthocyanins
Binding sites
Biosynthesis
Brassica
Oilseed crops
Oilseeds
Plant stress
Proteins
Rape plants
Rapeseed
Transcription factors
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Summary:CAPRICE (CPC) gene negatively regulates anthocyanin biosynthesis. It encodes a small single-repeat R3 MYB transcription factor, one of the seven of the kind, discovered in Arabidopsis. Oilseed rape (Brassica napus) is an economically valuable crop, a prospective object of genetic transformation and genome editing, and a close relative of Arabidopsis thaliana. Bioinformatic analysis showed that the gene CDS, as well as the resulting protein in Arabidopsis, are nearly twice larger than in oilseed rape. Two gRNAs were designed to knockout CPC-like genes of B. napus. Effectiveness of CRISPR/Cas9 genetic constructs were tested via agroinfiltration. It was demonstrated that CPC proteins of B. napus are involved in anthocyanin biosynthesis despite their smaller size. gRNA spacer, fully corresponding to the DNA-binding site, appeared to be critical for the knockout of the CPC gene. One of the generated genetic constructs can be used for genetic transformation of oilseed rape and the study of the role of anthocyanins in plant stress response.
ISSN:0094-243X
1551-7616
DOI:10.1063/5.0068528