The influence of cultivation conditions and strain of yeast on mannan polysaccharide content in cells

• Main Authors: Harbah, R. A., Meledina, T. V., Manshin, D. V., Morozov, A. A.
• Format: Conference Proceeding
• Language: English
• Subjects: Cultivation; Lager; Plates; Polysaccharides; Yeast
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0069264

The present study investigated the influence of cultivation conditions and the role of the yeast strains on the content of mannan in yeast cells. Two strains of S. cerevisiae were used, cultivated using two cultivation methods. The mannan content was determined at different stages of yeast growth. According to the obtained results, the highest mannan content was found in yeast S. cerevisiae Belgian Wit M21, which grew in batch culture (on a medium prepared from barley wort extract) at a temperature of 28 °C after 9 hours of cultivation, where its content increased by 98.98%. Whereas mannan content in the cells grown in a batch culture at 24 °C and the cells grown on Petri plates at temperature 28 °C was maximum after 15 hours of cultivation. Its content increased by 99.85% and 90.69%, respectively. In yeast S. cerevisiae Californian Lager M54, which was grown in the batch culture at a temperature of 28 °C, the highest amount of mannan in the cells was after 9 hours, where its content increased by 57.61%. In contrast, the highest amount of mannan in the cells grown on Petri plates at temperature 28 °C was obtained after 15 hours of cultivation, and its content increased by 32.47% in the cells.