Agroforensic, a new emerging study using molecular marker technique

• Main Authors: Listanto, Edy, Warsun, Ahmad, Dadang, Ahmad, Riyanti, Eny Ida, Pardal, Saptowo Jumali, Sustiprijatno, Mastur
• Format: Conference Proceeding
• Language: English
• Subjects: Cornflour; Deoxyribonucleic acid; DNA; Flour; Genetic fingerprinting; Markers; Qualitative analysis; Rice
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0075164

Agroforensic will identify and solve the problem when one case such as genetic diversity of crops should be declared, and in other situation need some confirmation like as detection of genetic modified organism (GMO) on food product or agrobusiness. Identification of DNA fingerprinting in forensics can be presented as DNA pattern profile containing repetitive and varied sequences among the investigated individuals. Several PCR-based technologies have been used in such genetic analysis. This study was conducted based on SSR analysis and GMO detection using a total of 13 SSR markers and 2 specific primers, respectively. The SSR samples consisted of 4 samples of sticky rice, 3 samples of brown rice, 8 samples of white rice, and 2 samples of corn, while GMO detection was conducted on 3 samples of corn flour. The aims of this study were to determine the genetic validity between the tested samples compared to the local control rice based on SSR analysis, to confirm the similarity of corn samples based on SSR analysis, and to identify the GMO content of corn flour sample based on qualitative PCR. The results of SSR analysis on sticky rice, brown rice, and white rice showed that their DNA band patterns were different from the local controls. This result was also supported by NTSys analysis. The results of SSR analysis on corn samples indicated that there were different DNA banding pattern between the control and sample corn based on six SSR primers. The results of GMO detection showed that three samples of corn flour were negative. Agroforensic can be performed using SSR method based on PCR technique.