Polyatomic Liquid Oxygen (PLO®): A new methodology for the production in aqueous solution of reactive oxygen and nitrogen species (RONS) to be applied in medical treatments

Free radicals play a pivotal role in cell physiology as “gaseous messengers/transmitters.” The radical superoxide (O2·−) and H2O2 molecules are called Reactive Oxygen Species (ROS); nitric oxide and peroxynitrite are named Reactive Nitrogen Species (RNS). All these species constitute an integrated c...

Full description

Saved in:
Bibliographic Details
Published in:AIP advances 2021-12, Vol.11 (12), p.125218-125218-9
Main Authors: Barco, Giovanni, Bramanti, Emilia, Onor, Massimo, Benedetti, Edoardo, Mameli, Marina, Mangano, Andrea, Pascone, Alessandro, Prati, Ubaldo
Format: Article
Language:English
Subjects:
Allotropy
Apoptosis
Aqueous solutions
Electron beams
Free radicals
Hydrogen peroxide
In vivo methods and tests
Liquid oxygen
Nitric oxide
Nitrogen
Nitrogen oxides
Physiology
Signalling systems
Stability
Transmitters
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Free radicals play a pivotal role in cell physiology as “gaseous messengers/transmitters.” The radical superoxide (O2·−) and H2O2 molecules are called Reactive Oxygen Species (ROS); nitric oxide and peroxynitrite are named Reactive Nitrogen Species (RNS). All these species constitute an integrated cellular signaling system. ROS and RNS act on cell proliferation, differentiation, migration, and apoptosis, thus becoming potential anticancer drugs. Because of their chemical instability and short half-life, they cannot be used directly. In this work, we describe an original methodology to produce an aqueous mixture of reactive oxygen and nitrogen species (RONS) in which the gas transmitter molecules derived from the dioxygen and nitrogen oxide have sufficient chemical stability, suitable for in vitro studies of cell physiology. This technique is based on the generation of an electron beam obtained through an inverse sputtering electron device. The result is a gaseous mixture of allotropes of both oxygen and nitrogen in trace amounts, later dissolved in an aqueous phase. This mixture is defined either with the acronym OPL® (Ossigeno Poliatomico Liquido) or PLO® (Polyatomic Liquid Oxygen) or OPL-RONS®. We report herein the chemical characterization of PLO. The stability of PLO makes it suitable for in vivo studies and medical applications.
ISSN:2158-3226
2158-3226
DOI:10.1063/5.0075895