Detection of insertion/deletion in MIF introns in Rheumatoid arthritis patients and healthy persons in Iraq

• Main Authors: Hussein, Ali Razzaq, Mejbel, Fatima Abdul Hussein, Aldujaili, Nawfal Hussein
• Format: Conference Proceeding
• Language: English
• Subjects: Arthritis; Autoimmune diseases; Cytokines; Deletion; Deoxyribonucleic acid; DNA; Electrophoresis; Females; Gene sequencing; Insertion; Males; Rheumatoid arthritis
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0093405

Rheumatoid arthritis (RA) is autoimmune disorder caused by variety of factor; one of these factors is genetic variation in cytokine as Macrophage Migration Inhibitory Factor (MIF). There is no study detect or relate insertion and/or deletion of MIF introns in RA. This study aimed to investigate the present and/or the contribution of 189bp (Intron 1) and 95bp (Intron 2) insertion / deletion allele of MIF introns in RA development. Genomic DNA was extracted from blood of 88 subjects: 44 RA patients (8 males and 36 females) and 44 control (23 males and 21 females). This study used primers to cover large fragment of MIF gene to give product of 272bp by conventional PCR. The PCR products of randomly selected five subjects (three RA and two controls) were sending to Macrogen (Korea) for DNA sequencing by Sanger method. RA was significantly (P=0.001) higher in 50s decades (38.6%) and female (81.82%). Moreover, the gel image of PCR detects no deletion but only insertion in MIF introns. The results of the PCR-Gel electrophoresis were confirmed by DNA sequencing, the results showed that all subjects contain sequences cover exon 1, intron 1, exon 2, intron 2 and exon 3 regions as that targeted by primer that give PCR product of 727bp after NCBI BLAST with Homo sapiens MIF (RefSeqGene on chromosome 22, Sequence ID: NG_012099.1). RA is age- and gender-dependent illness and there is insertion and no deletion in MIF Intron 1 and Intron 2 (confirmed by DNA sequencing) in RA and healthy Iraq population.