Label free deep penetration single photon microscopic imaging with ultralong anti-diffracting beam

Label free single photon microscopic imaging has natural advantages in noninvasive in vivo tissue imaging such as high resolution and rapid imaging speed. Although label free multi-photon microscopy can be used for imaging thick tissue samples, it requires high excitation light power and is phototox...

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Bibliographic Details
Published in:Applied physics letters 2022-07, Vol.121 (2)
Main Authors: Guo, Yong, Han, Hongyi, Wang, Luwei, Zhu, Yinru, Gao, Xinwei, Yang, Zhigang, Weng, Xiaoyu, Yan, Wei, Qu, Junle
Format: Article
Language:English
Subjects:
Applied physics
Biological properties
Chloroplasts
Excitation
Image resolution
Imaging techniques
Microscopy
Penetration depth
Photon beams
Photons
Scattering
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Summary:Label free single photon microscopic imaging has natural advantages in noninvasive in vivo tissue imaging such as high resolution and rapid imaging speed. Although label free multi-photon microscopy can be used for imaging thick tissue samples, it requires high excitation light power and is phototoxic to the samples. Conventional label free single photon microscopy requires lower excitation light power, but it has limited imaging depth. Observing some highly scattering thick tissue samples with single photon microscopy is a great challenge. To solve the problem, we developed a label free deep penetration single photon microscopic imaging technique with an ultralong anti-diffracting (UAD) beam. The penetrating ability of the UAD beam was verified by passing through turbid media and performed with autofluorescence of chloroplasts in fresh Epipremnum aureum leaves. Benefiting from the anti-diffracting properties and the elongated focal depth of the UAD beam, single photon UAD microscopy has deeper penetration depth and better anti-scattering ability and is one of the ideal methods to observe the deep structure of biological samples.
ISSN:0003-6951
1077-3118
DOI:10.1063/5.0097959