Assessment of multilocus sequences analysis (MLSA) for the identification of myxobacteria strains

Identification and classification of bacteria using 16S rRNA gene is unable to differentiate many strains in one species including member of myxobacteria. The aim in this study was to evaluated myxobacteria strains of the genus Myxococcus. Total five strains from intra- and inter-species were select...

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Bibliographic Details
Main Authors: Octaviana, Senlie, Mozef, Tjandrawati, Wink, Joachim
Format: Conference Proceeding
Language:English
Subjects:
Antiinfectives and antibacterials
Deoxyribonucleic acid
DNA
Evaluation
Genes
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Summary:Identification and classification of bacteria using 16S rRNA gene is unable to differentiate many strains in one species including member of myxobacteria. The aim in this study was to evaluated myxobacteria strains of the genus Myxococcus. Total five strains from intra- and inter-species were selected based on difference antimicrobial activities. The gyrB, pyrG, rpoB, pgm and the 16S rNA genes were used in the MLSA evaluation. The results highlighted the concatenated housekeeping genes which showed a clear distinction with high resolution of five strain of Myxococcus in phylogenetic and taxonomic studies. The MLSA provided a powerful tool to identify species and their phylogeny especially in highly genetic diversity study. To support the concatenated housekeeping genes similarity value among member of the order Myxococcales, it is recommended to analyze the DDH (DNA-DNA Hybridization) of strains.
ISSN:0094-243X
1551-7616
DOI:10.1063/5.0118330