Detection of thiamine in pharmaceutical supplement using differential pulse anodic stripping voltammetry

• Main Authors: Wahyuni, Wulan Tri, Putra, Budi Riza, Nisa, Ulfiatun, Rohaeti, Eti
• Format: Conference Proceeding
• Language: English
• Subjects: Anodic stripping; Deposition; Electrodes; Glassy carbon; Optimization; Parameters; Pharmaceuticals; Thiamine; Vitamin B; Voltammetry
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0133238

Given the importance of thiamine for health, a quick and easy method of thiamine detection is needed. This study at to develop a thiamine detection method using a glassy carbon electrode (GCE) with differential pulse anodic stripping voltammetry (DPASV) technique. The developed method for thiamine detection was then applied as thiamine protocol detection in the pharmaceutical supplement. Optimization parameters of the DPASV technique in detecting thiamine include pH of the electrolyte solution, precipitation potential, and precipitation time. Meanwhile, the analytical performance of the developed method for thiamine detection was investigated in several parameters, including linearity, precision, detection limit, and quantitation limit. The optimum conditions for thiamine detection using the DPASV technique were obtained at pH of 12, deposition potential of -1600 mV(vs Ag/AgCl), and a deposition time of 240 s. The analytical performance of glassy carbon electrode in thiamine detection has an R2 value of 0.994, %SBR of 4.5669%, limit of detection, and quantization Limit of 1.2 x 10−5 M and 1.59 x 10−5 M, respectively. This method can be applied for thiamine detection in vitamin B1 supplements with a percent accuracy of 95.96% and vitamin B complex supplements with a percent accuracy of 93.30%.