Molecular and morphological characterization of Vanilla and Pasak Bumi plants

Vanilla and Pasak Bumi were phytopharmaceutical plants from Indonesia that are important for medicinal purposes and industries. The morphological characterization alone of these plants is often caused misidentification which affected further effort to search their secondary metabolites product. Data...

Full description

Saved in:
Bibliographic Details
Main Authors: Kusumaningrum, Hermin Pancasakti, Dhanyaputri, Erizca, Kurniawati, Mufida Budi, Hermasanti, Maulidya Syafira, Dunya, Carmelita Nosa Bethari, Listyanto, Handika Arya, Jauhari, Muhammad Amien, Kirana, Salma Allea, Muhammad, Ibnu Maulvi, Mawarni, Shelina Nurun, Khairunnisa, Dinda, Erfianti, Tia, Eshananda, Yuriza, Nurhayati, Ferniah, Rejeki Siti, Jannah, Siti Nur
Format: Conference Proceeding
Language:English
Subjects:
Metabolites
Morphology
Polymerase chain reaction
Vanilla
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Vanilla and Pasak Bumi were phytopharmaceutical plants from Indonesia that are important for medicinal purposes and industries. The morphological characterization alone of these plants is often caused misidentification which affected further effort to search their secondary metabolites product. Data about morphological characters accomplished with molecular identification will be objective, more accurate and reliable. This study aims to morphologically and molecularly characterize Vanilla and Pasak Bumi plants using Internal transcribe Spacer region marker. The morphological characterization stage was carried out using a morphological key. Molecular characterization began with DNA isolation with CTAB, then DNA was amplified by polymerase chain reaction (PCR). DNA was then analyzed using the Basic Local Alignment Search Tool (BLAST). The research result showed the variation of morphological character of Vanilla and Pasak Bumi plant in Indonesia supported with the result of molecular identification.
ISSN:0094-243X
1551-7616
DOI:10.1063/5.0140209