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Construction of low humidity biosafety level-2 laboratory for cryo-sample environment for soft x-ray tomography imaging at Taiwan photon source

The facility of the low humidity laboratory with Biosafety Level 2 (BSL-2) was ready to prepare cryogenic samples for accessing soft X-ray tomography (SXT) at Taiwan Photon Source (TPS). The laboratory has many crucial functions, such as cryo-sample transferring, cryo-sample preparation, humidity bu...

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Bibliographic Details
Main Authors: Hsieh, Chia-Chun, Lin, Zi-Jing, Lai, Lee-Jene
Format: Conference Proceeding
Language:English
Subjects:
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Summary:The facility of the low humidity laboratory with Biosafety Level 2 (BSL-2) was ready to prepare cryogenic samples for accessing soft X-ray tomography (SXT) at Taiwan Photon Source (TPS). The laboratory has many crucial functions, such as cryo-sample transferring, cryo-sample preparation, humidity buffer, liquid nitrogen (LN2) filling, and dry air production, achieving these functions in five separate partitions. Furthermore, to meet the preparation for various biosamples, by setting a hardware and airflow direction for BSL-2 facility is constructed. This constructed laboratory has sufficient safety and reduces the moisture to an ambient relative humidity (RH) below 15%. Moreover, we show some successful SXT images that the cryo-sample was prepared and transferred using this laboratory.
ISSN:0094-243X
1551-7616
DOI:10.1063/5.0168153