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Construction of low humidity biosafety level-2 laboratory for cryo-sample environment for soft x-ray tomography imaging at Taiwan photon source

The facility of the low humidity laboratory with Biosafety Level 2 (BSL-2) was ready to prepare cryogenic samples for accessing soft X-ray tomography (SXT) at Taiwan Photon Source (TPS). The laboratory has many crucial functions, such as cryo-sample transferring, cryo-sample preparation, humidity bu...

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Main Authors:	Hsieh, Chia-Chun, Lin, Zi-Jing, Lai, Lee-Jene
Format:	Conference Proceeding
Language:	English
Subjects:	Air flow Liquid nitrogen Moisture effects Photons Relative humidity Soft x rays Tomography X ray imagery
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creator	Hsieh, Chia-Chun Lin, Zi-Jing Lai, Lee-Jene
description	The facility of the low humidity laboratory with Biosafety Level 2 (BSL-2) was ready to prepare cryogenic samples for accessing soft X-ray tomography (SXT) at Taiwan Photon Source (TPS). The laboratory has many crucial functions, such as cryo-sample transferring, cryo-sample preparation, humidity buffer, liquid nitrogen (LN2) filling, and dry air production, achieving these functions in five separate partitions. Furthermore, to meet the preparation for various biosamples, by setting a hardware and airflow direction for BSL-2 facility is constructed. This constructed laboratory has sufficient safety and reduces the moisture to an ambient relative humidity (RH) below 15%. Moreover, we show some successful SXT images that the cryo-sample was prepared and transferred using this laboratory.
doi_str_mv	10.1063/5.0168153
format	conference_proceeding
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identifier	ISSN: 0094-243X
ispartof	AIP conference proceedings, 2023, Vol.2990 (1)
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source	American Institute of Physics:Jisc Collections:Transitional Journals Agreement 2021-23 (Reading list)
subjects	Air flow Liquid nitrogen Moisture effects Photons Relative humidity Soft x rays Tomography X ray imagery
title	Construction of low humidity biosafety level-2 laboratory for cryo-sample environment for soft x-ray tomography imaging at Taiwan photon source
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