Study role of cytokine in patients with co-infection H. pylori and E. histolytica

• Main Authors: Toma, Rasha Saad, Al-Hadraawy, Saleem Khteer, Aljanaby, Ahmed Abduljabbar Jaloob
• Format: Conference Proceeding
• Language: English
• Subjects: Cytokines; Females; Infections; Injuries; Interleukins; Joint and ligament injuries; Males; Patients
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0182315

The study was conducted on350 suspected patients (192 male and 158 female) and 28 healthy people (14 male and 14 female) as the control group. Age ranged from 10-50 years old. All these cases were examined by the wet mount microscopic method to detect Entamoeba histolytica. Antigen rapid test to detect the Helicobacter pylori, all these samples collected from suspected patients were clients of a private laboratory suspected to be infected with H. pylori when attended to AL-Emmam AL-Sajaad hospital and AL-Forat AL-Awsat in AL-Najaf province from January 2022 till May 2023. Any patient was using the drug or undergoing disease removal from the current study. This study was designed to determine the effects of E. histolytica, H. pylori, and the two pathogens combined infection on parameters such as IL-10 and IL-13. The current study revealed that the concentration of (IL-10) in patient infection with Helicobacter pylori, Entamoeba histolytica and in joint injury Helicobacter pylori with Entamoeba histolytica were significant increase (P < 0.05) (272. 48 ± 12.33 ng/L), (818.81 ± 99.03 ng/L), (363.84 ± 47.40 ng/L) respectively in compared to the control group (216.18 ±18.52 ng/L), also it revealed the interleukin levels were highly elevated in samples infected with E. histolytica in comparison to those with H. pylori alone or combined with E. histolytica. Also, the current study revealed that the concentration of (IL-13) in patient infection with Helicobacter pylori, Entamoeba histolytica and in joint injury Helicobacter pylori with Entamoeba histolytica were significant increase (P < 0.05) (8. 1080 ± 1.40 ng/L), (14.658 ± 2.05 ng/L), (8. 607 ± 0.92 ng/L) respectively in compared to the control group (3.4259 ±0.65 ng/L), also it revealed the interleukin levels were highly elevated in samples infected with E. histolytica in comparison to those with H. pylori alone or combined with E. histolytica. The current study has concluded that the infection with Helicobacter pylori, Entamoeba histolytica and in joint injury, Helicobacter pylori with Entamoeba histolytica affects the body’s immunity represented by IL-10 and IL-13.