Investigation of some cytokines among patients with urinary tract infections caused by Providincia rettgeri

• Main Authors: Abdul-Hassan, Layla Saleh, Alkhudhairy, Miaad K., Al-Kraety, Israa Abdul Ameer
• Format: Conference Proceeding
• Language: English
• Subjects: Blood; Cytokines; Interleukins; Tumor necrosis factor-TNF; Urinary tract infections; Urine
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0182435

The objective of this research was to examine the relationships between Interleukin-8 (IL-8), Interleukin-6 (IL-6), and Tumor Necrosis Factor-alpha (TNF-) levels in Providencia rettgeri UTI, since UTI is one of the most typical bacterial infections. The methodology involves retrieving 139 urine and blood specimens from UTI patients with ages ranging from 15 to 55, and also another 100 samples from healthy volunteers to operate as a sample group. Every sample of urine was inoculated on blood agar and MacConkey agar plates with such a sterile loop and then incubated at 37 degrees Celsius in an aerobic environment for 24 hours. The proportion of human IL-8, IL-6, and TNF-α - was analyzed both in the study’s patient and treated subjects using an Enzyme-Linked Immunosorbent Assay (ELISA) kit. Approximately 5.03 percent of analyzed samples tested positive for P. rettgeri, in accordance with the results. The level of IL-8, IL-6 and TNF-α considerably were high inpatient which reached to (319.87±0.06, 292.99±0.01and112.45±0.91) respectively, as compared with contrast group (67.23±0.023, 71.56±0.035 and 61.27±0.026) respectively. Age, gender, and residence all had a role in the probability of contracting P. rettgeri. In conclusion, target cytokines have such a major role in UTI, especially during in the acute phase. In furthermore, there were substantial differences in gender, age and residence location among all those infected with P. rettgeri, and there are significant positive correlations between IL-8, IL-6, and TNF-α in P. rettgeri-infected individuals.