Calcium species in phloem sap of castor bean

Calcium species in phloem sap of castor bean was separated by preparative scale size exclusion chromatography (SEC) and the calcium content detected by inductively coupled plasma quadrupole mass spectrometry (ICP-QMS). A 500µL phloem sap sample of Castor bean was injected onto a Sephadex G-50 superf...

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Bibliographic Details
Main Authors: Fitri, Noor, Idris, Muhammad, Buchari
Format: Conference Proceeding
Language:English
Subjects:
Buffer solutions
Calcium
Inductively coupled plasma
Low molecular weights
Mass spectrometry
Quadrupoles
Size exclusion chromatography
Ultrafines
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Summary:Calcium species in phloem sap of castor bean was separated by preparative scale size exclusion chromatography (SEC) and the calcium content detected by inductively coupled plasma quadrupole mass spectrometry (ICP-QMS). A 500µL phloem sap sample of Castor bean was injected onto a Sephadex G-50 superfine (SF) column (700 mm x 24 mm) using 20 mM MES buffer solution pH 8.0 as a mobile phase with flow rate 0.3 mL/min. Calcium content in chromatographic fractions was determined by ICP-QMS. By this method, two peaks of calcium species were present at low molecular weight area (ℽ 1 kDa). This information is useful for knowing the process of transporting calcium species in plants.
ISSN:0094-243X
1551-7616
DOI:10.1063/5.0204762