Tomographic phase microscopy of living three-dimensional cell cultures

A successful application of self-interference digital holographic microscopy in combination with a sample-rotation-based tomography module for three-dimensional (3-D) label-free quantitative live cell imaging with subcellular resolution is demonstrated. By means of implementation of a hollow optical...

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Bibliographic Details
Published in:Journal of biomedical optics 2014-04, Vol.19 (4), p.046009-046009
Main Authors: Kuś, Arkadiusz, Dudek, Michał, Kemper, Björn, Kujawińska, Małgorzata, Vollmer, Angelika
Format: Article
Language:English
Subjects:
Biotechnology
Cell Culture Techniques - methods
Cell Line, Tumor
Clusters
Digital
Equipment Design
Holography - methods
Humans
Microscopy
Optical fibers
Organelles
Refractometry
Three dimensional
Tomography
Tomography, Optical - methods
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Summary:A successful application of self-interference digital holographic microscopy in combination with a sample-rotation-based tomography module for three-dimensional (3-D) label-free quantitative live cell imaging with subcellular resolution is demonstrated. By means of implementation of a hollow optical fiber as the sample cuvette, the observation of living cells in different 3-D matrices is enabled. The fiber delivers a stable and accurate rotation of a cell or cell cluster, providing quantitative phase data for tomographic reconstruction of the 3-D refractive index distribution with an isotropic spatial resolution. We demonstrate that it is possible to clearly distinguish and quantitatively analyze several cells grouped in a "3-D cluster" as well as subcellular organelles like the nucleoli and local internal refractive index changes.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.19.4.046009