Stimulus-evoked outer segment changes in rod photoreceptors

Rod-dominated transient retinal phototropism (TRP) has been recently observed in freshly isolated mouse and frog retinas. Comparative confocal microscopy and optical coherence tomography revealed that the TRP was predominantly elicited from the rod outer segment (OS). However, the biophysical mechan...

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Bibliographic Details
Published in:Journal of biomedical optics 2016-06, Vol.21 (6), p.065006-065006
Main Authors: Zhao, Xiaohui, Thapa, Damber, Wang, Benquan, Lu, Yiming, Gai, Shaoyan, Yao, Xincheng
Format: Article
Language:English
Subjects:
Animals
Anura
Light
Mice
Photoreceptor Cells, Vertebrate - radiation effects
Phototropism
Research Papers: General
Retina - radiation effects
Rod Cell Outer Segment - radiation effects
Time-Lapse Imaging
Tomography, Optical Coherence
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Summary:Rod-dominated transient retinal phototropism (TRP) has been recently observed in freshly isolated mouse and frog retinas. Comparative confocal microscopy and optical coherence tomography revealed that the TRP was predominantly elicited from the rod outer segment (OS). However, the biophysical mechanism of rod OS dynamics is still unknown. Mouse and frog retinal slices, which displayed a cross-section of retinal photoreceptors and other functional layers, were used to test the effect of light stimulation on rod OSs. Time-lapse microscopy revealed stimulus-evoked conformational changes of rod OSs. In the center of the stimulated region, the length of the rod OS shrunk, while in the peripheral region, the rod OS swung toward the center region. Our experimental observation and theoretical analysis suggest that the TRP may reflect unbalanced rod disc-shape changes due to localized visible light stimulation.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.21.6.065006