Inactivation of Burkholderia pseudomalleibsaQ results in decreased invasion efficiency and delayed escape of bacteria from endocytic vesicles

Burkholderia pseudomallei , an infectious Gram-negative bacterium, is the causative pathogen of melioidosis. In the present study, a B. pseudomallei strain with mutation in the bsaQ gene, encoding a structural component of the type III secretion system (T3SS), was constructed. This bsaQ mutation cau...

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Bibliographic Details
Published in:Archives of microbiology 2008, Vol.190 (6), p.623-631
Main Authors: Muangsombut, Veerachat, Suparak, Supaporn, Pumirat, Pornpan, Damnin, Suwat, Vattanaviboon, Paiboon, Thongboonkerd, Visith, Korbsrisate, Sunee
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
Ecology
Life Sciences
Microbial Ecology
Microbiology
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Summary:Burkholderia pseudomallei , an infectious Gram-negative bacterium, is the causative pathogen of melioidosis. In the present study, a B. pseudomallei strain with mutation in the bsaQ gene, encoding a structural component of the type III secretion system (T3SS), was constructed. This bsaQ mutation caused a marked decrease in secretion of BopE effector and BipD translocator proteins into culture supernatant. The B. pseudomallei bsaQ mutant also exhibited decreased efficiencies of plaque formation, invasion into non-phagocytic cells and multinucleated giant cell (MNGC) development in a J774A.1 macrophage cell line. Co-localization of the bacteria and lysosome-associated membrane glycoprotein-1 (LAMP-1) containing vesicles suggested that defects in MNGC formation may result from the delayed ability of this B. pseudomallei mutant to escape from the vacuoles of macrophages.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-008-0413-3