Carotenoid production and gene expression in an astaxanthin-overproducing Xanthophyllomycesdendrorhous mutant strain

The primary carotenoid synthesized by Xanthophyllomyces dendrorhous is astaxanthin, which is used as a feed additive in aquaculture. Cell growth kinetics and carotenoid production were correlated with the mRNA levels of the idi , crtE , crtYB , crtI , crtS and crtR genes, and the changes in gene seq...

Full description

Saved in:
Bibliographic Details
Published in:Archives of microbiology 2015-09, Vol.197 (10), p.1129-1139
Main Authors: Castelblanco-Matiz, Lina M., Barbachano-Torres, Alejandra, Ponce-Noyola, Teresa, Ramos-Valdivia, Ana C., Cerda García-Rojas, Carlos M., Flores-Ortiz, César M., Barahona-Crisóstomo, Salvador K., Baeza-Cancino, Marcelo E., Alcaíno-Gorman, Jennifer, Cifuentes-Guzmán, Víctor H.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
Ecology
Life Sciences
Microbial Ecology
Microbiology
Original Paper
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The primary carotenoid synthesized by Xanthophyllomyces dendrorhous is astaxanthin, which is used as a feed additive in aquaculture. Cell growth kinetics and carotenoid production were correlated with the mRNA levels of the idi , crtE , crtYB , crtI , crtS and crtR genes, and the changes in gene sequence between the wild-type and a carotenoid overproducer XR4 mutant strain were identified. At the late stationary phase, the total carotenoid content in XR4 was fivefold higher than that of the wild-type strain. Additionally, the mRNA levels of crtE and crtS increased during the XR4 growth and were three times higher than the wild-type strain in the late stationary phase. Moreover, the nucleotide sequences of crtYB , crtI and crtR exhibited differences between the strains. Both the higher crtE and crtS transcript levels and the crtYB , crtI and crtR mutations can, at least in part, act to up-regulate the carotenoid biosynthesis pathway in the XR4 strain.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-015-1153-9