Bioaccumulation, resistance, and remediation of Mn(II) and Cu(II) and their impacts on antioxidant enzymes of Anoxybacillusflavithermus

• Published in: International journal of environmental science and technology (Tehran) 2023-10, Vol.20 (10), p.10823-10834
• Main Authors: Özdemir, S., Turkan, Z., Kılınc, E., Altuner, E. E., Sen, F.
• Format: Article
• Language: English
• Subjects: Aquatic Pollution; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Science and Engineering; Original Paper; Soil Science & Conservation; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 1735-1472; 1735-2630
• DOI: 10.1007/s13762-023-05133-y

In this article, the effects of copper (Cu(II)) and manganese (Mn(II)) ions on the superoxide dismutase (SOD) and catalase enzymes (CAT) of the thermophilic Anoxybacillus flavithermus (A. flavithermus) were thoroughly examined. Results from experiments using the minimum inhibitory concentration (MIC) showed that A. flavithermus was less tolerant of ions in the liquid media than in the solid medium. In comparison to the control, the greatest percentage of bacterial growth was determined to be 20.4% including the addition of Mn(II) and 17.6% including the addition of Cu(II). Mn(II) bioaccumulation increases the total weight of the bacteria, while Cu(II) bioaccumulation specifically increases the weight of dry A. flavithermus (MDBW). The ability of A. flavithermus cell membrane to bioaccumulate ions was examined. At various ion concentrations, the CAT and SOD activities were examined. Any possible morphological alteration following the encounter with ions. The findings suggested that A. flavithermus may be used to extract and remove hazardous metals from industrial wastewaters. A. flavithermus is a potential bioindicator of hazardous metal-contaminated waters, according to antioxidative enzyme activity. Additionally, the activities of the antioxidant enzymes CAT and SOD increased with increasing ion concentrations up to 10.0 mg/L over a 48 h period. This indicates a protective response against oxidative stress caused by Cu(II) and Mn(II) ions.