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Inhibition of fungal disease development in plants by engineering controlled cell death
We have tested a new strategy for engineering fungal disease resistance in crops which is based on inhibition of fungal growth and reproduction by controlled generation of necrotic lesions at infection sites, analogous to the naturally occurring hypersensitive cell death. The approach relies on the...
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| Published in: | Bio/Technology 1995-10, Vol.13 (10), p.1085-1089 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c417t-8552eb318dbbed90cfbca227f0c18c0bb51f06c32dfdfa8e72512890d89158233 |
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| cites | cdi_FETCH-LOGICAL-c417t-8552eb318dbbed90cfbca227f0c18c0bb51f06c32dfdfa8e72512890d89158233 |
| container_end_page | 1089 |
| container_issue | 10 |
| container_start_page | 1085 |
| container_title | Bio/Technology |
| container_volume | 13 |
| creator | Strittmatter, Günter Janssens, Jan Opsomer, Chris Botterman, Johan |
| description | We have tested a new strategy for engineering fungal disease resistance in crops which is based on inhibition of fungal growth and reproduction by controlled generation of necrotic lesions at infection sites, analogous to the naturally occurring hypersensitive cell death. The approach relies on the use of two chimeric genes. On the one hand, a promoter fragment of the potato
prp1-1
gene which mediates rapid and localized transcriptional activation selectively in response to pathogen attack is used to drive the expression of the bacterial ribonuclease, barnase. On the other hand, transgenic plants express barstar, a specific inhibitor of barnase, to minimize the detrimental effects of potential background barnase synthesis in non-infected tissue. Sporulation of the late-blight fungus
Phytophthora infestans
was considerably reduced on leaves from transgenic potato lines harboring this two-component system. Treatment of leaves from such plants with ethylene, a chemical stimulus of
prp1-1
promoter activity, resulted in rapid tissue destruction, indirectly indicating the inducibility and action of barnase. Furthermore, the reduction of fungal sporulation cosegregated with the transgenes in an F1 population. |
| doi_str_mv | 10.1038/nbt1095-1085 |
| format | article |
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prp1-1
gene which mediates rapid and localized transcriptional activation selectively in response to pathogen attack is used to drive the expression of the bacterial ribonuclease, barnase. On the other hand, transgenic plants express barstar, a specific inhibitor of barnase, to minimize the detrimental effects of potential background barnase synthesis in non-infected tissue. Sporulation of the late-blight fungus
Phytophthora infestans
was considerably reduced on leaves from transgenic potato lines harboring this two-component system. Treatment of leaves from such plants with ethylene, a chemical stimulus of
prp1-1
promoter activity, resulted in rapid tissue destruction, indirectly indicating the inducibility and action of barnase. Furthermore, the reduction of fungal sporulation cosegregated with the transgenes in an F1 population.</description><identifier>ISSN: 0733-222X</identifier><identifier>EISSN: 2331-3684</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt1095-1085</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>adn recombinado ; adn recombine ; Agriculture ; bacillus ; Bioinformatics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; disease resistance ; enzyme inhibitors ; esporulacion ; ethylene ; etileno ; Fundamental and applied biological sciences. Psychology ; gene ; gene transfer ; genes ; Genetic engineering ; Genetic technics ; genetic transformation ; genetica ; genetics ; genetique ; inhibicion ; inhibidores de enzimas ; inhibiteur d' enzyme ; inhibition ; Life Sciences ; Methods. Procedures. Technologies ; necrose ; necrosis ; pathogenese ; pathogenesis ; patogenesis ; Phytophthora infestans ; plantas transgenicas ; plante transgenique ; proteinas ; proteine ; proteins ; recombinant dna ; resistance aux maladies ; resistencia a la enfermedad ; ribonucleasas ; ribonuclease ; ribonucleases ; solanum ; Solanum tuberosum ; sporulation ; transferencia de genes ; transfert de gene ; transformacion genetica ; transformation genetique ; Transgenic animals and transgenic plants ; Transgenic plants</subject><ispartof>Bio/Technology, 1995-10, Vol.13 (10), p.1085-1089</ispartof><rights>Nature Publishing Company 1995</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-8552eb318dbbed90cfbca227f0c18c0bb51f06c32dfdfa8e72512890d89158233</citedby><cites>FETCH-LOGICAL-c417t-8552eb318dbbed90cfbca227f0c18c0bb51f06c32dfdfa8e72512890d89158233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2894599$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Strittmatter, Günter</creatorcontrib><creatorcontrib>Janssens, Jan</creatorcontrib><creatorcontrib>Opsomer, Chris</creatorcontrib><creatorcontrib>Botterman, Johan</creatorcontrib><creatorcontrib>Max-Plank-Institut fur Zuchtungsforschung, Koln, Germany</creatorcontrib><creatorcontrib>Universidad Autonoma Chapingo, Chapingo, Mex. (Mexico). Departamento de Fitotecnia</creatorcontrib><title>Inhibition of fungal disease development in plants by engineering controlled cell death</title><title>Bio/Technology</title><addtitle>Nat Biotechnol</addtitle><description>We have tested a new strategy for engineering fungal disease resistance in crops which is based on inhibition of fungal growth and reproduction by controlled generation of necrotic lesions at infection sites, analogous to the naturally occurring hypersensitive cell death. The approach relies on the use of two chimeric genes. On the one hand, a promoter fragment of the potato
prp1-1
gene which mediates rapid and localized transcriptional activation selectively in response to pathogen attack is used to drive the expression of the bacterial ribonuclease, barnase. On the other hand, transgenic plants express barstar, a specific inhibitor of barnase, to minimize the detrimental effects of potential background barnase synthesis in non-infected tissue. Sporulation of the late-blight fungus
Phytophthora infestans
was considerably reduced on leaves from transgenic potato lines harboring this two-component system. Treatment of leaves from such plants with ethylene, a chemical stimulus of
prp1-1
promoter activity, resulted in rapid tissue destruction, indirectly indicating the inducibility and action of barnase. Furthermore, the reduction of fungal sporulation cosegregated with the transgenes in an F1 population.</description><subject>adn recombinado</subject><subject>adn recombine</subject><subject>Agriculture</subject><subject>bacillus</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>disease resistance</subject><subject>enzyme inhibitors</subject><subject>esporulacion</subject><subject>ethylene</subject><subject>etileno</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene</subject><subject>gene transfer</subject><subject>genes</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>genetic transformation</subject><subject>genetica</subject><subject>genetics</subject><subject>genetique</subject><subject>inhibicion</subject><subject>inhibidores de enzimas</subject><subject>inhibiteur d' enzyme</subject><subject>inhibition</subject><subject>Life Sciences</subject><subject>Methods. Procedures. Technologies</subject><subject>necrose</subject><subject>necrosis</subject><subject>pathogenese</subject><subject>pathogenesis</subject><subject>patogenesis</subject><subject>Phytophthora infestans</subject><subject>plantas transgenicas</subject><subject>plante transgenique</subject><subject>proteinas</subject><subject>proteine</subject><subject>proteins</subject><subject>recombinant dna</subject><subject>resistance aux maladies</subject><subject>resistencia a la enfermedad</subject><subject>ribonucleasas</subject><subject>ribonuclease</subject><subject>ribonucleases</subject><subject>solanum</subject><subject>Solanum tuberosum</subject><subject>sporulation</subject><subject>transferencia de genes</subject><subject>transfert de gene</subject><subject>transformacion genetica</subject><subject>transformation genetique</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><issn>0733-222X</issn><issn>2331-3684</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNp1kc1LHTEUxYNU6Kt217WEUlx1bD7mI1kWsa0guFBpdyHJ3Dwj85JpkhH8781jHtJNV3fzu-eecy5Cnyi5oISLb8EUSmTXUCK6I7RhnNOG96J9hzZk4LxhjP15jz7k_ERIO_Ss3aDf1-HRG198DDg67Jaw1RMefQadAY_wDFOcdxAK9gHPkw4lY_OCIWx9AEg-bLGNoaQ4TTBiC1NdBl0eT9Gx01OGj4d5gh5-XN1f_mpubn9eX36_aWxLh9KIrmNgOBWjMTBKYp2xmrHBEUuFJcZ01JHecja60WkBA-soE5KMQtJO1IAn6HzVnVP8u0Auaufz3oYOEJes6EBYS2Rfwa8raFPMOYFTc_I7nV4UJWrfnjq0p_btVfzLQVdnqyeXdLA-v-1UD20nZcWaFcvzvgtI6ikuKdTE_5P9vPJBlyXBm96_n6vQ2Qo5HZXepnr34Y5K2RPSi0EI_gpNGJPS</recordid><startdate>19951001</startdate><enddate>19951001</enddate><creator>Strittmatter, Günter</creator><creator>Janssens, Jan</creator><creator>Opsomer, Chris</creator><creator>Botterman, Johan</creator><general>Nature Publishing Group US</general><general>Nature Publications</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>19951001</creationdate><title>Inhibition of fungal disease development in plants by engineering controlled cell death</title><author>Strittmatter, Günter ; Janssens, Jan ; Opsomer, Chris ; Botterman, Johan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-8552eb318dbbed90cfbca227f0c18c0bb51f06c32dfdfa8e72512890d89158233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>adn recombinado</topic><topic>adn recombine</topic><topic>Agriculture</topic><topic>bacillus</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>disease resistance</topic><topic>enzyme inhibitors</topic><topic>esporulacion</topic><topic>ethylene</topic><topic>etileno</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene</topic><topic>gene transfer</topic><topic>genes</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>genetic transformation</topic><topic>genetica</topic><topic>genetics</topic><topic>genetique</topic><topic>inhibicion</topic><topic>inhibidores de enzimas</topic><topic>inhibiteur d' enzyme</topic><topic>inhibition</topic><topic>Life Sciences</topic><topic>Methods. Procedures. Technologies</topic><topic>necrose</topic><topic>necrosis</topic><topic>pathogenese</topic><topic>pathogenesis</topic><topic>patogenesis</topic><topic>Phytophthora infestans</topic><topic>plantas transgenicas</topic><topic>plante transgenique</topic><topic>proteinas</topic><topic>proteine</topic><topic>proteins</topic><topic>recombinant dna</topic><topic>resistance aux maladies</topic><topic>resistencia a la enfermedad</topic><topic>ribonucleasas</topic><topic>ribonuclease</topic><topic>ribonucleases</topic><topic>solanum</topic><topic>Solanum tuberosum</topic><topic>sporulation</topic><topic>transferencia de genes</topic><topic>transfert de gene</topic><topic>transformacion genetica</topic><topic>transformation genetique</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Strittmatter, Günter</creatorcontrib><creatorcontrib>Janssens, Jan</creatorcontrib><creatorcontrib>Opsomer, Chris</creatorcontrib><creatorcontrib>Botterman, Johan</creatorcontrib><creatorcontrib>Max-Plank-Institut fur Zuchtungsforschung, Koln, Germany</creatorcontrib><creatorcontrib>Universidad Autonoma Chapingo, Chapingo, Mex. (Mexico). Departamento de Fitotecnia</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bio/Technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Strittmatter, Günter</au><au>Janssens, Jan</au><au>Opsomer, Chris</au><au>Botterman, Johan</au><aucorp>Max-Plank-Institut fur Zuchtungsforschung, Koln, Germany</aucorp><aucorp>Universidad Autonoma Chapingo, Chapingo, Mex. (Mexico). Departamento de Fitotecnia</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of fungal disease development in plants by engineering controlled cell death</atitle><jtitle>Bio/Technology</jtitle><stitle>Nat Biotechnol</stitle><date>1995-10-01</date><risdate>1995</risdate><volume>13</volume><issue>10</issue><spage>1085</spage><epage>1089</epage><pages>1085-1089</pages><issn>0733-222X</issn><eissn>2331-3684</eissn><eissn>1546-1696</eissn><abstract>We have tested a new strategy for engineering fungal disease resistance in crops which is based on inhibition of fungal growth and reproduction by controlled generation of necrotic lesions at infection sites, analogous to the naturally occurring hypersensitive cell death. The approach relies on the use of two chimeric genes. On the one hand, a promoter fragment of the potato
prp1-1
gene which mediates rapid and localized transcriptional activation selectively in response to pathogen attack is used to drive the expression of the bacterial ribonuclease, barnase. On the other hand, transgenic plants express barstar, a specific inhibitor of barnase, to minimize the detrimental effects of potential background barnase synthesis in non-infected tissue. Sporulation of the late-blight fungus
Phytophthora infestans
was considerably reduced on leaves from transgenic potato lines harboring this two-component system. Treatment of leaves from such plants with ethylene, a chemical stimulus of
prp1-1
promoter activity, resulted in rapid tissue destruction, indirectly indicating the inducibility and action of barnase. Furthermore, the reduction of fungal sporulation cosegregated with the transgenes in an F1 population.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><doi>10.1038/nbt1095-1085</doi><tpages>5</tpages></addata></record> |
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| ispartof | Bio/Technology, 1995-10, Vol.13 (10), p.1085-1089 |
| issn | 0733-222X 2331-3684 1546-1696 |
| language | eng |
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| subjects | adn recombinado adn recombine Agriculture bacillus Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology disease resistance enzyme inhibitors esporulacion ethylene etileno Fundamental and applied biological sciences. Psychology gene gene transfer genes Genetic engineering Genetic technics genetic transformation genetica genetics genetique inhibicion inhibidores de enzimas inhibiteur d' enzyme inhibition Life Sciences Methods. Procedures. Technologies necrose necrosis pathogenese pathogenesis patogenesis Phytophthora infestans plantas transgenicas plante transgenique proteinas proteine proteins recombinant dna resistance aux maladies resistencia a la enfermedad ribonucleasas ribonuclease ribonucleases solanum Solanum tuberosum sporulation transferencia de genes transfert de gene transformacion genetica transformation genetique Transgenic animals and transgenic plants Transgenic plants |
| title | Inhibition of fungal disease development in plants by engineering controlled cell death |
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