STIMULATING EFFECTS OF MULTIWALLED CARBON NANOTUBES ON IMMOBILIZED GLUCONACETOBACTER SUCROFERMENTAS VKPM B-11267 CELLS

• Published in: Nanotechnologies in Russia 2020, Vol.15 (1), p.79-82
• Main Authors: Tarasov, S. E., Plekhanova, Yu. V., Bykov, A. G., Klenova, N. A., Reshetilov, A. N.
• Format: Article
• Language: English
• Subjects: Chemistry and Materials Science; Devices and Products Based on Nanomaterials and Nanotechnologies; Industrial and Production Engineering; Machines; Manufacturing; Materials Science; Nanotechnology; Processes
• ISSN: 1995-0780; 1995-0799
• DOI: 10.1134/S1995078020010127

The Gluconacetobacter sucrofermentas bacterial cells are immobilized in a chitosan gel on the surface of an electrode obtained by matrix printing. The nature of the change in the bioelectrochemical parameters of the immobilized bacteria under the influence of multiwalled carbon nanotubes (MWCNTs) are studied. We studied the electrochemical signals of cells upon the introduction of substrates (glucose, ethanol) by three methods: chronoamperometry, cyclic voltammetry, and impedance spectroscopy. The introduction of ethyl alcohol at a concentration of 1 mM leads to a decrease in the total resistance of the electrode by a factor of ~40. Modification of the electrode using MWCNTs leads to an increase in the current (by factors of 8 to 10) and an additional decrease in the total electrode resistance by a factor of ~10. These effects are similar to the effects of substrates and MWCNTs on the phylogenetically related Gluconobacter oxydans cells. The formation of bacterial cellulose (BC) membrane cells during the growth does not fundamentally change the effects of the substrates and MWCNTs.