Is acetylcholine an autocrine/paracrine growth factor via the nicotinic α7-receptor subtype in the human colon cancer cell line HT-29?

We used immunochemistry to demonstrate expression of acetylcholine's nicotinic α7-receptor subtype in human colon cancer cell line HT-29. Moreover, RT-PCR and immunochemistry showed that choline acetyltransferase and acetylcholine esterase, the enzymes responsible for acetylcholine synthesis an...

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Bibliographic Details
Published in:European journal of pharmacology 2009-05, Vol.609 (1), p.27-33
Main Authors: Pettersson, Ann, Nilsson, Linn, Nylund, Gunnar, Khorram-Manesh, Amir, Nordgren, Svante, Delbro, Dick S.
Format: Article
Language:English
Subjects:
Acetylcholine
Acetylcholine esterase
Alpha7
Biological and medical sciences
Biomedical Sciences
Biomedicinsk vetenskap
Choline acetyltransferase
Gastroenterology. Liver. Pancreas. Abdomen
HT-29 human colon cancer cell line
Medical sciences
Nicotinic receptor
Pharmacology. Drug treatments
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
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Summary:We used immunochemistry to demonstrate expression of acetylcholine's nicotinic α7-receptor subtype in human colon cancer cell line HT-29. Moreover, RT-PCR and immunochemistry showed that choline acetyltransferase and acetylcholine esterase, the enzymes responsible for acetylcholine synthesis and degradation, respectively, localise in HT-29 cells. Bromoacetylcholine bromide, an inhibitor of choline acetyltransferase, significantly attenuated basal cell growth. Our findings suggest that acetylcholine might serve as an autocrine/paracrine–or speculatively, even intracrine–signalling molecule in cell line HT-29, thus contributing to carcinogenesis/cancer progression.
ISSN:0014-2999
1879-0712
1879-0712
DOI:10.1016/j.ejphar.2009.03.002