Monolithic methacrylate packed 96-tips for high throughput bioanalysis

In the pharmaceutical industry the growing number of samples to be analyzed requires high throughput and fully automated analytical techniques. Commonly used sample-preparation methods are solid-phase extraction (SPE), liquid–liquid extraction (LLE) and protein precipitation. In this paper we will d...

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Bibliographic Details
Published in:Journal of Chromatography A 2010-04, Vol.1217 (16), p.2581-2588
Main Authors: Altun, Zeki, Skoglund, Christina, Abdel-Rehim, Mohamed
Format: Article
Language:English
Subjects:
Automated sample preparation
Chemistry
Kemi
LC–MS/MS
Methacrylate monolith
Packed 96-tips
Plasma and blood samples
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Summary:In the pharmaceutical industry the growing number of samples to be analyzed requires high throughput and fully automated analytical techniques. Commonly used sample-preparation methods are solid-phase extraction (SPE), liquid–liquid extraction (LLE) and protein precipitation. In this paper we will discus a new sample-preparation technique based on SPE for high throughput drug extraction developed and used by our group. This new sample-preparation method is based on monolithic methacrylate polymer as packing sorbent for 96-tip robotic device. Using this device a 96-well plate could be handled in 2–4 min. The key aspect of the monolithic phase is that monolithic material can offer both good binding capacity and low back-pressure properties compared to e.g. silica phases. The present paper presents the successful application of monolithic 96-tips and LC–MS/MS by the sample preparation of busulphan, rescovitine, metoprolol, pindolol and local anaesthetics from human plasma samples and cyklophosphamid from mice blood samples.
ISSN:0021-9673
DOI:10.1016/j.chroma.2009.10.072