Visual Detection of DNA on Paper Chips

On-site DNA analysis for diagnostic or forensic purposes is much anticipated in the future of molecular testing. Yet the challenges to achieve this goal remain large with rapid and inexpensive detection and visualization being key factors for any portable analysis system. We have developed a filter...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2014-02, Vol.86 (3), p.1575-1582
Main Authors: Song, Yajing, Gyarmati, Péter, Araújo, Ana Catarina, Lundeberg, Joakim, Brumer, Harry, Ståhl, Patrik L
Format: Article
Language:English
Subjects:
Analysis system
Analytical chemistry
Animals
Beads
Bioassays
Biochemistry
Capillary force
Chips
Cost-efficient
Deoxyribonucleic acid
Diagnostic systems
DNA
DNA - analysis
DNA - chemistry
DNA - isolation & purification
DNA Probes - chemistry
Dogs
Filter paper
Filter paper material
Filtration - instrumentation
Forensic engineering
Forensic Sciences
Genomics
Humans
Instrumentation
Mitochondria
Nucleic acid assays
On-site detection
Species Specificity
Surface Properties
Time Factors
Visual detection
Visualization
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Summary:On-site DNA analysis for diagnostic or forensic purposes is much anticipated in the future of molecular testing. Yet the challenges to achieve this goal remain large with rapid and inexpensive detection and visualization being key factors for any portable analysis system. We have developed a filter paper-based nucleic acid assay, which is able to identify and distinguish dog and human genomic and mitochondrial samples in a forensic setting. The filter paper material allows for transport by capillary force of the sample DNA through the detection surface, allowing the targets to hybridize specifically to their complementary capture sequences. Coupling micrometer-sized beads to DNA allows the results to be visualized by the naked eye, enabling instant, cost-efficient, and on-site detection, while eliminating the need for advanced expensive instrumentation.
ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/ac403196b