Capillary electrophoretic and mass spectrometric analysis of a polydisperse fluorosurfactant

A fluorosurfactant has been studied using capillary electrophoresis and mass spectrometry. The fluorosurfactant, FC134, can be used as a buffer additive in capillary electrophoresis in order to decrease wall adsorption of proteins and in micellar electrokinetic chromatography. However, it has been d...

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Bibliographic Details
Published in:Journal of separation science 2005-02, Vol.28 (3), p.239-244
Main Authors: Al-Jarah, Suhair Yousif, Sjödahl, Johan, Woldegiorgis, Andreas, Emmer, Åsa
Format: Article
Language:English
Subjects:
Analytical chemistry
anionic surfactants
basic-proteins
Capillary electrophoresis
cationic surfactants
Chemistry
Chromatographic methods and physical methods associated with chromatography
chromatography
ESI-MS
ethoxylates
Exact sciences and technology
Fractionation
General and physical chemistry
indirect photometric detection
MALDI-MS
matrix
nonionic surfactants
Other chromatographic methods
Polydisperse fluorosurfactant
separation
Surface physical chemistry
Surface-active agents: properties
time-of-flight
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Summary:A fluorosurfactant has been studied using capillary electrophoresis and mass spectrometry. The fluorosurfactant, FC134, can be used as a buffer additive in capillary electrophoresis in order to decrease wall adsorption of proteins and in micellar electrokinetic chromatography. However, it has been discovered that this fluorosurfactant is polydisperse, thus containing substances with different lengths and structures. In this work, the fluorosurfactant sample components were separated by capillary electrophoresis. An uncoated as well as a poly(vinyl alcohol)‐coated capillary were used with running electrolytes containing methanol and acetic acid. Following the capillary electrophoretic separation, fractions were collected for further analysis by MALDI‐MS. Non‐fractionated samples were also analyzed both by MALDI‐MS and by ESI‐MS.
ISSN:1615-9306
1615-9314
1615-9314
DOI:10.1002/jssc.200400040