Point-of-care vertical flow allergen microarray assay: proof of concept

Sophisticated equipment, lengthy protocols, and skilled operators are required to perform protein microarray-based affinity assays. Consequently, novel tools are needed to bring biomarkers and biomarker panels into clinical use in different settings. Here, we describe a novel paper-based vertical fl...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2014-09, Vol.60 (9), p.1209-1216
Main Authors: Chinnasamy, Thiruppathiraja, Segerink, Loes I, Nystrand, Mats, Gantelius, Jesper, Andersson Svahn, Helene
Format: Article
Language:English
Subjects:
Accuracy
Allergens
Allergens - analysis
Allergies
Binders
Colorimetry
Data processing
Flow control
Gold
Gold - chemistry
Humans
Immunoassay
Immunoglobulin E
Immunoglobulin E - blood
Laboratories
Membranes
Metal Nanoparticles
Metal Nanoparticles - chemistry
Nanoparticles
Point-of-Care Systems
Protein Array Analysis
Proteins
Reagents
Reproducibility of Results
Sensitivity and Specificity
Vertical flow
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Summary:Sophisticated equipment, lengthy protocols, and skilled operators are required to perform protein microarray-based affinity assays. Consequently, novel tools are needed to bring biomarkers and biomarker panels into clinical use in different settings. Here, we describe a novel paper-based vertical flow microarray (VFM) system with a multiplexing capacity of at least 1480 microspot binding sites, colorimetric readout, high sensitivity, and assay time of
ISSN:0009-9147
1530-8561
1530-8561
DOI:10.1373/clinchem.2014.223230