Quantum Rod Bioconjugates as Targeted Probes for Confocal and Two-Photon Fluorescence Imaging of Cancer Cells

Live cell imaging using CdSe/CdS/ZnS quantum rods (QRs) as targeted optical probes is reported. The QRs, synthesized in organic media using a binary surfactant mixture, were dispersed in aqueous media using mercaptoundecanoic acid (MUA) and lysine. Transferrin (Tf) was linked to the QRs to produce Q...

Full description

Saved in:
Bibliographic Details
Published in:Nano letters 2007-03, Vol.7 (3), p.761-765
Main Authors: Yong, Ken-Tye, Qian, Jun, Roy, Indrajit, Lee, Hoon Hi, Bergey, Earl J, Tramposch, Kenneth M, He, Sailing, Swihart, Mark T, Maitra, Anirban, Prasad, Paras N
Format: Article
Language:English
Subjects:
Biological and medical sciences
cdse nanocrystals
dots
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
General aspects, investigation technics, apparatus
growth
HeLa Cells
Humans
Microscopy, Confocal
Microscopy, Electron
Microscopy, Fluorescence, Multiphoton
Molecular Probes - chemistry
nanorods
Nanotechnology
Nanotubes - chemistry
Nanotubes - ultrastructure
Quantum Dots
shape control
size
Tissues, organs and organisms biophysics
Transferrin - pharmacokinetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Live cell imaging using CdSe/CdS/ZnS quantum rods (QRs) as targeted optical probes is reported. The QRs, synthesized in organic media using a binary surfactant mixture, were dispersed in aqueous media using mercaptoundecanoic acid (MUA) and lysine. Transferrin (Tf) was linked to the QRs to produce QR-Tf bioconjugates that were used for targeted in vitro delivery to a human cancer cell line. Confocal and two-photon imaging were used to confirm receptor-mediated uptake of QR-Tf conjugates into the HeLa cells, which overexpress the transferrin receptor (TfR). Uptake was not observed with QRs that lacked Tf functionalization or with cells that were presaturated with free Tf and then treated with Tf-functionalized QRs.
ISSN:1530-6984
1530-6992
1530-6992
DOI:10.1021/nl063031m