Bioactive Surface Modification of Metal Oxides via Catechol-Bearing Modular Peptides: Multivalent-Binding, Surface Retention, and Peptide Bioactivity

• Published in: Journal of the American Chemical Society 2014-11, Vol.136 (46), p.16357-16367
• Main Authors: Tang, Wen, Policastro, Gina M, Hua, Geng, Guo, Kai, Zhou, Jinjun, Wesdemiotis, Chrys, Doll, Gary L, Becker, Matthew L
• Format: Article
• Language: English
• Subjects: 3T3 Cells; Adsorption; Alkaline Phosphatase - metabolism; Animals; Binding affinities; Binding energy; Bioactive glass; Bioactive peptides; Biochemistry; Bone sialoproteins; Catechols - chemistry; Cell Adhesion - drug effects; Cell culture; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Dendrimers; Dendrimers - chemistry; Histones - chemistry; Histones - pharmacology; Hydrogen-Ion Concentration; Immobilized Proteins - chemistry; Immobilized Proteins - pharmacology; Inorganic materials; Intercellular Signaling Peptides and Proteins - chemistry; Intercellular Signaling Peptides and Proteins - pharmacology; Mice; Minerals - metabolism; Orthopaedic implants; Osteogenesis - drug effects; Osteogenic growth peptides; Phenols; Quartz; Quartz crystal microbalance with dissipation; Quartz crystal microbalances; Solid phase synthesis; Surface Properties; Synthesis (chemical); Titanium - chemistry; Titanium dioxide
• ISSN: 0002-7863; 1520-5126; 1520-5126
• DOI: 10.1021/ja508946h

A series of multivalent dendrons containing a bioactive osteogenic growth peptide (OGP) domain and surface-binding catechol domains were obtained through solid phase synthesis, and their binding affinity to hydroxyapatite, TiO2, ZrO2, CeO2, Fe3O4 and gold was characterized using a quartz crystal microbalance with dissipation (QCM-d). Using the distinct difference in binding affinity of the bioconjugate to the metal oxides, TiO2-coated glass slides were selectively patterned with bioactive peptides. Cell culture studies demonstrated the bioavailability of the OGP and that OGP remained on the surface for at least 2 weeks under in vitro cell culture conditions. Bone sialoprotein (BSP) and osteocalcein (OCN) markers were upregulated 3-fold and 60-fold, respectively, relative to controls at 21 days. Similarly, 3-fold more calcium was deposited using the OGP tethered dendron compared to TiO2. These catechol-bearing dendrons provide a fast and efficient method to functionalize a wide range of inorganic materials with bioactive peptides and have the potential to be used in coating orthopaedic implants and fixation devices.