Application of novel bacterial consortium for biodegradation of aromatic amine 2-ABS using response surface methodology

• Published in: Journal of microbiological methods 2020-07, Vol.174, p.105941, Article 105941
• Main Authors: Fatima, Masoom, Saeed, Muhammad, Aslam, Muhammad, Lindström, Rakel Wreland, Farooq, Robina
• Format: Article
• Language: English
• Subjects: 2-aminobenzenesulfonic acid; Aerobic degradation; Bacterial consortium; COD removal; Response surface methodology
• ISSN: 0167-7012; 1872-8359; 1872-8359
• DOI: 10.1016/j.mimet.2020.105941

There is a strong need to develop purification methods for textile industrial wastewater containing toxic azo dyes. The reductive cleavage of azo dyes can be made by anaerobic bacteria, but the products of aromatic amines require an aerobic process. In this study a novel bacterial dye degrading consortium (DDC) of five isolated strains identified with 16S rRNA sequence: Proteus mirabilis (KR732288), Bacillus anthracis (KR732289), Enterobacter hormaechei (KR732290), Pseudomonas aeruginosa (KR732293) and Serratia rubidaea (KR732296) were used to aerobically decompose metabolite 2-aminobenxenesulfonic acid (2-ABS), as a model compound. The effect of three variables: temperature (28–42 °C), pH (5.0–8.0) and initial concentration of 2-ABS (5–40 ppm) was investigated in terms of degradation and chemical oxygen demand (COD) removal. Central composite design matrixand response surface methodology (RSM) were used for experimental design to evaluate theinteraction of the three process variables. The results show that up to 95% degradation and COD 90% removal are possible at optimal values of 32.4 ppm 2-ABS, pH 6.6 and a temperature of 35.7 °C. The theoretical response variables predicted by the developed RSM model was supported the experimental results. The optimized degradation of 2-ABS and COD removal were further confirmed by UV-HPLC analysis. [Display omitted] •Novel DDC consortium was developed for biodegradation of 2-ABS, a dye pollutant.•DDC include Proteus mirabilis, Bacillus anthracis, Enterobacter hormaechei, Pseudomonas aeruginosa and Serratia rubidaea.•An optimized aerobic degradation was achieved by adjusting process parameters statistically using RSM method.•The Full factorial 23 central composite design matrix have been used to design of experiments.•The high regression coefficient between variables and responses ensure the second-order polynomial regression model.•Maximum degradation of 2-ABS was upto 95% and COD removal was 90% in 6 days.