Scalable Production of Monodisperse Bioactive Spider Silk Nanowires

Elongated protein‐based micro‐ and nanostructures are of great interest for a wide range of biomedical applications, where they can serve as a backbone for surface functionalization and as vehicles for drug delivery. Current production methods for protein constructs lack precise control of either sh...

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Bibliographic Details
Published in:Macromolecular bioscience 2023-04, Vol.23 (4), p.e2200450-n/a
Main Authors: Gustafsson, Linnea, Kvick, Mathias, Åstrand, Carolina, Ponsteen, Nienke, Dorka, Nicolai, Hegrová, Veronika, Svanberg, Sara, Horák, Josef, Jansson, Ronnie, Hedhammar, My, van der Wijngaart, Wouter
Format: Article
Language:English
Subjects:
Animals
Biocompatibility
Biological activity
Biomedical materials
Biotechnology
Bioteknologi
cell aggregates
Cell culture
Cell Culture Techniques
Centrifugation
Drug delivery
Fabrication
Humans
liquid bridge induced assembly
Liquid bridges
Medical research
Mesenchymal stem cells
Nanostructures
Nanotechnology
Nanowires
Production methods
Proteins
scalable production
Silk
Silk - chemistry
spider silk
Spiders
Stem cells
Substrates
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Summary:Elongated protein‐based micro‐ and nanostructures are of great interest for a wide range of biomedical applications, where they can serve as a backbone for surface functionalization and as vehicles for drug delivery. Current production methods for protein constructs lack precise control of either shape and dimensions or render structures fixed to substrates. This work demonstrates production of recombinant spider silk nanowires suspended in solution, starting with liquid bridge induced assembly (LBIA) on a substrate, followed by release using ultrasonication, and concentration by centrifugation. The significance of this method lies in that it provides i) reproducability (standard deviation of length
ISSN:1616-5187
1616-5195
1616-5195
DOI:10.1002/mabi.202200450