Laboratory soft-x-ray microscope for cryotomography of biological specimens

Soft-x-ray cryotomography allows quantitative and high-resolution three-dimensional imaging of intact unstained cells. To date, the method relies on synchrotron-radiation sources, which limits accessibility for researchers. Here we present a laboratory water-window microscope for cryotomography. It...

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Bibliographic Details
Published in:Optics letters 2011-07, Vol.36 (14), p.2728-2730
Main Authors: BERTILSON, Michael, HOFSTEN, Olov Von, VOGT, Ulrich, HOLMBERG, Anders, CHRISTAKOU, Athanasia E, HERTZ, Hans M
Format: Article
Language:English
Subjects:
B-Lymphocytes - cytology
Computed-Tomography
Engineering physics
Exact sciences and technology
Fundamental areas of phenomenology (including applications)
HEK293 Cells
Humans
Kidney - cytology
Laboratories
Microscopy - methods
Optical physics
Optics
Optisk fysik
Physics
Resolution
Saccharomyces cerevisiae - cytology
TECHNOLOGY
TEKNIKVETENSKAP
Teknisk fysik
Tomography - methods
X-Rays
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Summary:Soft-x-ray cryotomography allows quantitative and high-resolution three-dimensional imaging of intact unstained cells. To date, the method relies on synchrotron-radiation sources, which limits accessibility for researchers. Here we present a laboratory water-window microscope for cryotomography. It is based on a λ=2.48 nm liquid-jet laser-plasma source, a normal-incidence multilayer condenser, a 30 nm zone-plate objective, and a cryotilt sample holder. We demonstrate high-resolution imaging, as well as quantitative tomographic imaging, of frozen intact cells. The reconstructed tomogram of the intracellular local absorption coefficient shows details down to ∼100 nm.
ISSN:0146-9592
1539-4794
1539-4794
DOI:10.1364/ol.36.002728