Electro-Osmotic Flow Through Closed-Open-Closed Microchannels: An Approach to Hyphenation of Capillary Electrophoresis and Maldi

We suggest electro-osmotic driven flow (EOF) through closed-open-closed microchannels as a novel approach for spatial sample separation using capillary electrophoresis (CE) prior to matrix assisted laser desorption/ionization mass spectroscopy (MALDI-MS). For this purpose we built a system consistin...

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Bibliographic Details
Main Authors: Parmar, V., Redeby, T., van der Wijngaart, W., Emmer, A., Stemme, G.
Format: Conference Proceeding
Language:English
Subjects:
Analytical chemistry
Analytisk kemi
Assisted-laser-desorption/ionization
Chemical analysis
Chemical technology
Chemistry
Electrokinetics
Ionization
Irrigation
Kemi
Mass spectroscopy
mass-spectrometry
Microchannel
Microfluidics
NATURAL SCIENCES
NATURVETENSKAP
Peptides
Silicon compounds
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Description
Summary:We suggest electro-osmotic driven flow (EOF) through closed-open-closed microchannels as a novel approach for spatial sample separation using capillary electrophoresis (CE) prior to matrix assisted laser desorption/ionization mass spectroscopy (MALDI-MS). For this purpose we built a system consisting of the series coupling of a closed fused silica capillary for separation, a microfabricated open microcanal for future MS detection and a second closed fused silica capillary for downstream liquid collection. This work verifies the EOF transport of a peptide sample in such a system with low dispersion.
ISSN:1084-6999
DOI:10.1109/MEMSYS.2006.1627822