Improvement in cell capture throughput using parallel bioactivated microfluidic channels

Optimization of targeted cell capture with microfluidic devices continues to be a challenge. On the one hand, microfluidics allow working with microliter volumes of liquids, whereas various applications in the real world require detection of target analyte in large volumes, such as capture of rare c...

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Bibliographic Details
Published in:Biomedical microdevices 2012-08, Vol.14 (4), p.625-629
Main Authors: Javanmard, Mehdi, Babrzadeh, Farbod, Nyrén, Pål, Davis, Ronald W.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Immobilized - chemistry
Antibodies, Immobilized - metabolism
Biological and Medical Physics
Biomedical Engineering and Bioengineering
Biomedical research
Biophysics
Candida albicans - cytology
Cell capture
Cell Separation - instrumentation
Cellular biology
Dimethylpolysiloxanes - chemistry
Engineering
Engineering Fluid Dynamics
Fluid dynamics
Microbiology
Microfluidic Analytical Techniques - instrumentation
Microfluidics
Nanotechnology
Pathogen detection
Pathogens
Saccharomyces cerevisiae - cytology
Time Factors
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Summary:Optimization of targeted cell capture with microfluidic devices continues to be a challenge. On the one hand, microfluidics allow working with microliter volumes of liquids, whereas various applications in the real world require detection of target analyte in large volumes, such as capture of rare cell types in several ml of blood. This contrast of volumes (microliter vs. ml) has prevented the emergence of microfluidic cell capture sensors in the clinical setting. Here, we study the improvement in cell capture and throughput achieved using parallel bioactivated microfluidic channels. The device consists of channels in parallel with each other tied to a single channel. We discuss fabrication and testing of our devices, and show the ability for an improvement in throughput detection of target cells.
ISSN:1387-2176
1572-8781
1572-8781
DOI:10.1007/s10544-012-9643-x