Surface functionalisation of carbon for low cost fabrication of highly stable electrochemical DNA sensors

An alternative strategy for surface tethering of DNA probes, where highly reactive glassy carbon (GC) substrates are prepared via electrochemical hydrogenation and electrochemical/chemical chlorination is reported. Thiolated DNA probes and alkanethiols were stably immobilised on the halogenated carb...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2015-09, Vol.71, p.25-29
Main Authors: Debela, Ahmed M., Ortiz, Mayreli, Beni, Valerio, O'Sullivan, Ciara K.
Format: Article
Language:English
Subjects:
Accessibility
Base Sequence
Biosensing Techniques - instrumentation
Biosensors
Carbon
Carbon - chemistry
Chlorination
Deoxyribonucleic acid
DNA - analysis
DNA Probes - chemistry
Electrochemical chlorination
Electrochemical DNA sensors
Electrochemical hydrogenation
Electrochemical Techniques - instrumentation
Equipment Design
Glassy carbon
Halogenation
Lateral spacing
Molecular Sequence Data
Nucleic Acid Hybridization
Sensors
Spacers
Sulfhydryl Compounds - chemistry
Surface chemistry
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Summary:An alternative strategy for surface tethering of DNA probes, where highly reactive glassy carbon (GC) substrates are prepared via electrochemical hydrogenation and electrochemical/chemical chlorination is reported. Thiolated DNA probes and alkanethiols were stably immobilised on the halogenated carbon, with electrochemical chlorination being milder, thus producing less damage to the surface. Electrochemical DNA sensors prepared using this surface chemistry on carbon with electrochemical chlorination providing an improved performance, producing a highly ordered surface and the use of lateral spacers to improve steric accessibility to immobilised probes was not required. [Display omitted] •Electrochemical hydrogenation and chemical/electrochemical halogenation exploited for surface tethering of thiolated DNA probes with application in electrochemical DNA sensors.
ISSN:0956-5663
1873-4235
1873-4235
DOI:10.1016/j.bios.2015.03.076