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Surface functionalisation of carbon for low cost fabrication of highly stable electrochemical DNA sensors
An alternative strategy for surface tethering of DNA probes, where highly reactive glassy carbon (GC) substrates are prepared via electrochemical hydrogenation and electrochemical/chemical chlorination is reported. Thiolated DNA probes and alkanethiols were stably immobilised on the halogenated carb...
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| Published in: | Biosensors & bioelectronics 2015-09, Vol.71, p.25-29 |
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| cites | cdi_FETCH-LOGICAL-c530t-51a61e2f854c98a37a3b4ea213489a92628ae933eddf6a79f12105b3da958a733 |
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| container_title | Biosensors & bioelectronics |
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| creator | Debela, Ahmed M. Ortiz, Mayreli Beni, Valerio O'Sullivan, Ciara K. |
| description | An alternative strategy for surface tethering of DNA probes, where highly reactive glassy carbon (GC) substrates are prepared via electrochemical hydrogenation and electrochemical/chemical chlorination is reported. Thiolated DNA probes and alkanethiols were stably immobilised on the halogenated carbon, with electrochemical chlorination being milder, thus producing less damage to the surface. Electrochemical DNA sensors prepared using this surface chemistry on carbon with electrochemical chlorination providing an improved performance, producing a highly ordered surface and the use of lateral spacers to improve steric accessibility to immobilised probes was not required.
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•Electrochemical hydrogenation and chemical/electrochemical halogenation exploited for surface tethering of thiolated DNA probes with application in electrochemical DNA sensors. |
| doi_str_mv | 10.1016/j.bios.2015.03.076 |
| format | article |
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•Electrochemical hydrogenation and chemical/electrochemical halogenation exploited for surface tethering of thiolated DNA probes with application in electrochemical DNA sensors.</description><identifier>ISSN: 0956-5663</identifier><identifier>ISSN: 1873-4235</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2015.03.076</identifier><identifier>PMID: 25880835</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Accessibility ; Base Sequence ; Biosensing Techniques - instrumentation ; Biosensors ; Carbon ; Carbon - chemistry ; Chlorination ; Deoxyribonucleic acid ; DNA - analysis ; DNA Probes - chemistry ; Electrochemical chlorination ; Electrochemical DNA sensors ; Electrochemical hydrogenation ; Electrochemical Techniques - instrumentation ; Equipment Design ; Glassy carbon ; Halogenation ; Lateral spacing ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Sensors ; Spacers ; Sulfhydryl Compounds - chemistry ; Surface chemistry</subject><ispartof>Biosensors & bioelectronics, 2015-09, Vol.71, p.25-29</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-51a61e2f854c98a37a3b4ea213489a92628ae933eddf6a79f12105b3da958a733</citedby><cites>FETCH-LOGICAL-c530t-51a61e2f854c98a37a3b4ea213489a92628ae933eddf6a79f12105b3da958a733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27915,27916</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25880835$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-118306$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Debela, Ahmed M.</creatorcontrib><creatorcontrib>Ortiz, Mayreli</creatorcontrib><creatorcontrib>Beni, Valerio</creatorcontrib><creatorcontrib>O'Sullivan, Ciara K.</creatorcontrib><title>Surface functionalisation of carbon for low cost fabrication of highly stable electrochemical DNA sensors</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>An alternative strategy for surface tethering of DNA probes, where highly reactive glassy carbon (GC) substrates are prepared via electrochemical hydrogenation and electrochemical/chemical chlorination is reported. Thiolated DNA probes and alkanethiols were stably immobilised on the halogenated carbon, with electrochemical chlorination being milder, thus producing less damage to the surface. Electrochemical DNA sensors prepared using this surface chemistry on carbon with electrochemical chlorination providing an improved performance, producing a highly ordered surface and the use of lateral spacers to improve steric accessibility to immobilised probes was not required.
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•Electrochemical hydrogenation and chemical/electrochemical halogenation exploited for surface tethering of thiolated DNA probes with application in electrochemical DNA sensors.</description><subject>Accessibility</subject><subject>Base Sequence</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensors</subject><subject>Carbon</subject><subject>Carbon - chemistry</subject><subject>Chlorination</subject><subject>Deoxyribonucleic acid</subject><subject>DNA - analysis</subject><subject>DNA Probes - chemistry</subject><subject>Electrochemical chlorination</subject><subject>Electrochemical DNA sensors</subject><subject>Electrochemical hydrogenation</subject><subject>Electrochemical Techniques - instrumentation</subject><subject>Equipment Design</subject><subject>Glassy carbon</subject><subject>Halogenation</subject><subject>Lateral spacing</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Sensors</subject><subject>Spacers</subject><subject>Sulfhydryl Compounds - chemistry</subject><subject>Surface chemistry</subject><issn>0956-5663</issn><issn>1873-4235</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkU1vEzEQhi0EoqHwBzggH7ns4o-115a4RC1fUgUHPq7WrHfcOHLiYO9S9d-zUUqOqKeZw_O-Gs1DyGvOWs64frdth5hrKxhXLZMt6_UTsuKml00npHpKVswq3Sit5QV5UeuWMdZzy56TC6GMYUaqFYnf5xLAIw3z3k8x7yHFCseF5kA9lGHZQi405Tvqc51ogKFEf0Y28XaT7mmdYEhIMaGfSvYb3C1Motdf17TivuZSX5JnAVLFVw_zkvz8-OHH1efm5tunL1frm8YryaZGcdAcRTCq89aA7EEOHYLgsjMWrNDCAFopcRyDht4GLjhTgxzBKgO9lJekOfXWOzzMgzuUuINy7zJEdx1_rV0uty7F2XFuJNML__bEH0r-PWOd3C5WjynBHvNcHe97JpnplH0EKpXlorOPaNVGd1zrji2oOKG-5FoLhvPJnLmjaLd1R9HuKNox6RbRS-jNQ_887HA8R_6ZXYD3JwCXX_-JWFz1Efcex1gWR27M8X_9fwGfWbm4</recordid><startdate>20150915</startdate><enddate>20150915</enddate><creator>Debela, Ahmed M.</creator><creator>Ortiz, Mayreli</creator><creator>Beni, Valerio</creator><creator>O'Sullivan, Ciara K.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SP</scope><scope>7U5</scope><scope>L7M</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>DG8</scope></search><sort><creationdate>20150915</creationdate><title>Surface functionalisation of carbon for low cost fabrication of highly stable electrochemical DNA sensors</title><author>Debela, Ahmed M. ; 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Thiolated DNA probes and alkanethiols were stably immobilised on the halogenated carbon, with electrochemical chlorination being milder, thus producing less damage to the surface. Electrochemical DNA sensors prepared using this surface chemistry on carbon with electrochemical chlorination providing an improved performance, producing a highly ordered surface and the use of lateral spacers to improve steric accessibility to immobilised probes was not required.
[Display omitted]
•Electrochemical hydrogenation and chemical/electrochemical halogenation exploited for surface tethering of thiolated DNA probes with application in electrochemical DNA sensors.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>25880835</pmid><doi>10.1016/j.bios.2015.03.076</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biosensors & bioelectronics, 2015-09, Vol.71, p.25-29 |
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| source | ScienceDirect Freedom Collection |
| subjects | Accessibility Base Sequence Biosensing Techniques - instrumentation Biosensors Carbon Carbon - chemistry Chlorination Deoxyribonucleic acid DNA - analysis DNA Probes - chemistry Electrochemical chlorination Electrochemical DNA sensors Electrochemical hydrogenation Electrochemical Techniques - instrumentation Equipment Design Glassy carbon Halogenation Lateral spacing Molecular Sequence Data Nucleic Acid Hybridization Sensors Spacers Sulfhydryl Compounds - chemistry Surface chemistry |
| title | Surface functionalisation of carbon for low cost fabrication of highly stable electrochemical DNA sensors |
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