Chicken seminal fluid lacks CD9‐ and CD44‐bearing extracellular vesicles

The avian seminal fluid (SF) is a protein‐rich fluid, derived from the testis, the rudimentary epididymis and, finally, from the cloacal gland. The SF interacts with spermatozoa and the inner cell lining of the female genital tract, to modulate sperm functions and female immune responsiveness. Its c...

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Bibliographic Details
Published in:Reproduction in domestic animals 2020-03, Vol.55 (3), p.293-300
Main Authors: Alvarez‐Rodriguez, Manuel, Ntzouni, Maria, Wright, Dominic, Khan, Kabirul Islam, López‐Béjar, Manel, Martinez, Cristina A., Rodriguez‐Martinez, Heriberto
Format: Article
Language:English
Subjects:
CD44
CD44 antigen
CD9
CD9 antigen
Chemical precipitation
Electron microscopy
Epididymis
exosome precipitation
Extracellular vesicles
Flow cytometry
Genital tract
Mammals
Membranes
Poultry
Proteomes
rooster
Semen
Seminal fluid
Seminal vesicle
Sperm
Spermatozoa
tetraspanins
Ultrastructure
Vesicles
western blot
Western blotting
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Summary:The avian seminal fluid (SF) is a protein‐rich fluid, derived from the testis, the rudimentary epididymis and, finally, from the cloacal gland. The SF interacts with spermatozoa and the inner cell lining of the female genital tract, to modulate sperm functions and female immune responsiveness. Its complex proteome might either be free or linked to extracellular vesicles (EVs) as it is the case in mammals, where EVs depict the tetraspanin CD9; and where those EVs derived from the epididymis (epididymosomes) also present the receptor CD44. In the present study, sperm‐free SF from Red Jungle Fowl, White Leghorn and an advanced intercross (AIL, 12th generation) were studied using flow cytometry of the membrane marker tetraspanin CD9, Western blotting of the membrane receptor CD44 and electron microscopy in non‐enriched (whole SF) or enriched fractions obtained by precipitation using a commercial kit (Total Exosome Precipitation Solution). Neither CD9‐ nor CD44 could be detected, and the ultrastructure confirmed the relative absence of EVs, raising the possibility that avian SF interacts differently with the female genitalia as compared to the seminal plasma of mammals.
ISSN:0936-6768
1439-0531
1439-0531
DOI:10.1111/rda.13617