Prediction of the response to antiangiogenic sunitinib therapy by non-invasive hybrid diffuse optics in renal cell carcinoma

• Published in: Biomedical optics express 2024-10, Vol.15 (10), p.5773-5789
• Main Authors: Mireles, Miguel, Jiménez-Valerio, Gabriela, Morales-Dalmau, Jordi, Johansson, Johannes D, Martínez-Lozano, Mar, Vidal-Rosas, Ernesto E, Navarro-Pérez, Valentí, Busch, David R, Casanovas, Oriol, Durduran, Turgut, Vilches, Clara
• Format: Article
• Language: English
• ISSN: 2156-7085; 2156-7085
• DOI: 10.1364/BOE.532052

In this work, broadband diffuse reflectance spectroscopy (DRS) and diffuse correlation spectroscopy (DCS) were used to quantify deep tissue hemodynamics in a patient-derived orthotopic xenograft mouse model of clear cell renal cancer undergoing antiangiogenic treatment. A cohort of twenty-two mice were treated with sunitinib and compared to thirteen control untreated mice, and monitored by DRS/DCS. A reduction in total hemoglobin concentration (THC, p = 0.03), oxygen saturation (SO p = 0.03) and blood flow index (BFI, p = 0.02) was observed over the treatment course. Early changes in tumor microvascular blood flow and total hemoglobin concentration were correlated with the final microvessel density (p = 0.014) and tumor weight (p = 0.024), respectively. Higher pre-treatment tumor microvascular blood flow was observed in non-responder mice with respect to responder mice, which was statistically predictive of the tumor intrinsic resistance (p = 0.01). This hybrid diffuse optical technique provides a method for predicting tumor intrinsic resistance to antiangiogenic therapy and could be used as predictive biomarker of response to antiangiogenic therapies in pre-clinical models.