Regional mapping of suppressor loci for anchorage independence and tumorigenicity on human chromosome 9

By microcell-mediated chromosome transfer to the malignant Syrian hamster cell line BHK-191-5C, we previously identified two suppressor functions on human chromosome 9 (HSA9), one for anchorage independence and another for tumorigenicity. However, the precise chromosomal locations of these suppresso...

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Bibliographic Details
Published in:Cancer genetics and cytogenetics 2001-10, Vol.130 (2), p.118-126
Main Authors: Eklund, Lena K., Islam, Khaleda, Söderkvist, Peter, Islam, M.Quamrul
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Chromosome Painting
Chromosomes, Human, Pair 9 - ultrastructure
Cricetinae
Databases as Topic
Foods and miscellaneous
Gene Deletion
Genes, Tumor Suppressor
Genetic Markers
Humans
In Situ Hybridization, Fluorescence
Medical sciences
MEDICIN
MEDICINE
Mesocricetus
Microsatellite Repeats
Models, Genetic
Phenotype
Polymerase Chain Reaction
Tumors
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Summary:By microcell-mediated chromosome transfer to the malignant Syrian hamster cell line BHK-191-5C, we previously identified two suppressor functions on human chromosome 9 (HSA9), one for anchorage independence and another for tumorigenicity. However, the precise chromosomal locations of these suppressor functions were not determined. The present study was undertaken to define the regional location of these suppressor loci using a panel of microcell hybrids containing structurally altered HSA9 with different deleted regions in the BHK-191-5C background. DNA derived from the cell hybrids was analyzed by PCR for verification of the presence of HSA9 genetic material by amplifying 62 microsatellite markers and 13 genes, covering the entire length of HSA9. Our deletion mapping data on anchorage independent and tumorigenic hybrids suggest that the suppressor function for anchorage independence is located in the region between 9q32 to 9qter. The suppressor for tumorigenicity may be located in one of three deleted regions on HSA9, the first one between the markers D9S162 and D9S1870, the second one between the markers D9S1868 and TIGRA002I21, and the third one between the markers D9S59 and D9S155.
ISSN:0165-4608
1873-4456
1873-4456
DOI:10.1016/S0165-4608(01)00471-X