Electronic nose for estimation of product concentration in mammalian cell cultivation

The off-gas composition from perfusion cultivation of a CHO-cell line producing recombinant human blood coagulation Factor VIII is monitored with an electronic nose. It is shown that the electronic nose in combination with an artificial neural network can be used for on-line estimation of the Factor...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2000, Vol.23 (6), p.637-642
Main Authors: BACHINGER, Th, RIESE, U, ERIKSSON, R. K, MANDENIUS, C. F
Format: Article
Language:English
Subjects:
Animal cells
Biological and medical sciences
Biotechnology
coagulation factor VIII
Establishment of new cell lines, improvement of cultural methods, mass cultures
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
NATURAL SCIENCES
NATURVETENSKAP
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Summary:The off-gas composition from perfusion cultivation of a CHO-cell line producing recombinant human blood coagulation Factor VIII is monitored with an electronic nose. It is shown that the electronic nose in combination with an artificial neural network can be used for on-line estimation of the Factor VIII concentration in production-scale cultivations. The obtained prediction error (1 ) for the Factor VIII concentration was 1.1 IU/ml. The potential of the electronic nose for estimation of viable cell count is outlined in laboratory-scale Factor VIII cultivations. The obtained prediction error (1 ) for the viable cell count was 0.4x10 super(6) cells/ml. The results show that this non-invasive method is potentially useful for on-line bioprocess monitoring.
ISSN:0178-515X
1615-7591
1432-0797
1432-0797
DOI:10.1007/s004490000213